辅助生殖技术致妊娠的早期流产绒毛染色体异常分析

发布时间：2018-10-11 08:43

【摘要】：目的探讨辅助生殖技术致妊娠的早期流产绒毛染色体异常情况。方法将622例孕早期流产的孕妇分为两组,其中辅助生殖技术致妊娠的早期流产孕妇156例为ART组,自然妊娠早期流产孕妇466例为对照组。分别对两组孕妇的绒毛细胞进行绒毛细胞分离、培养及染色体分析。结果对照组正常核型299例,异常核型167例,染色体异常类型最多见的是染色体数量异常,以47,XX/XY,+22、47,XX/XY,+16多见,其他可见三体和嵌合体;ART组正常核型87例,异常核型69例,以常染色体三体最多见共计33例,最多见的三体为47,XX/XY,+22、47,XX/XY,+16,三倍体型4例、染色体多态性2例、嵌合体型3例、单体型4例、四倍体型2例;对照组不同年龄(40岁、≥40岁)间染色体异常率差异有统计学意义(P0.05),ART组不同年龄(40岁、≥40岁)间染色体异常率差异有统计学意义(P0.05),ART组与对照组的染色体异常率差异无统计学意义(P0.05)。结论与自然妊娠相比,辅助生殖技术妊娠并不增加孕早期自然流产胚胎染色体异常的发生率,染色体异常与年龄有关。
[Abstract]:Objective to investigate the chromosomal abnormalities of villi in early abortion induced by assisted reproductive technique. Methods 622 pregnant women with early abortion were divided into two groups: ART group (ART group) and control group (466 cases). The chorionic villi of two groups of pregnant women were isolated, cultured and chromosome analyzed. Results in the control group, there were 299 normal karyotypes and 167 abnormal karyotypes. The most common chromosomal abnormalities were chromosome quantitative abnormalities, with 47 / XXXY, 22 / 47 / XXXXXY, 16, and other trisomy and chimerism, while 87 normal karyotypes and 69 abnormal karyotypes were found in the ART group. There were 33 cases of autosomal trisomy, the most common trisomy was 47XXXXXY, 2247xr XY, 16s, triploid 4, chromosome polymorphism 2, chimera 3, haplotype 4, tetraploid 2, control group 40 years old. There was significant difference in chromosomal abnormality rate between), ART group and control group (P0.05). There was no significant difference between), ART group and control group (P0.05). Conclusion compared with natural pregnancy, assisted reproductive pregnancy does not increase the incidence of chromosomal abnormalities in embryos of early spontaneous abortion, and chromosomal abnormalities are related to age.
【作者单位】：广州医科大学附属第三医院妇产科研究所实验部广东省产科重大疾病重点实验室广东省普通高校生殖与遗传重点实验室;
【分类号】：R714.21

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