瘦素基因甲基化水平与妊娠期糖尿病的相关性

发布时间：2018-10-15 11:54

【摘要】：目的分析妊娠期糖尿病(GDM)孕妇及糖耐量正常(NGT)孕妇瘦素及其基因启动子区甲基化的动态变化水平,以进一步探索妊娠期糖尿病发病机制。方法采用放射免疫法检测45例(GDM组20例,NGT组25例)孕妇妊娠晚期血清瘦素水平;逆转录PCR(RT-PCR)及实时荧光定量PCR(Real-time PCR)检测两组孕妇胎盘组织中瘦素mRNA的表达,并进一步应用蛋白印迹法(Western blot)检测瘦素蛋白的表达。利用氧化亚硫酸盐测序法及亚硫酸盐测序法检测两组孕妇瘦素基因启动子区甲基化及羟甲基化水平。结果 GDM组孕妇妊娠晚期血清瘦素水平明显高于NGT组(P0.05);GDM孕妇胎盘组织瘦素mRNA及瘦素蛋白表达水平高于NGT组;两组孕妇瘦素基因启动子区均发生甲基化及羟甲基化,GDM组甲基化水平高于NGT组(P0.05);两组孕妇部分位点羟甲基化水平存在差异,但无统计学意义。结论妊娠期糖尿病孕妇瘦素水平高于糖耐量正常孕妇。瘦素基因启动子区甲基化动态变化过程与妊娠期糖尿病的发病有一定相关性。
[Abstract]:Objective to analyze the dynamic changes of methylation of leptin and its gene promoter in (GDM) pregnant women with gestational diabetes mellitus (GDM) and normal glucose tolerance (NGT) women in order to explore the pathogenesis of gestational diabetes mellitus (GDM). Methods Serum leptin levels were measured by radioimmunoassay in 45 pregnant women (GDM 20, NGT 25), reverse transcription PCR (RT-PCR) and real-time quantitative PCR (Real-time PCR) were used to detect the expression of leptin mRNA in placenta of pregnant women. Furthermore, the expression of leptin protein was detected by Western blot (Western blot). The methylation and hydroxymethylation of leptin gene promoter in two groups of pregnant women were detected by oxidative sulfite sequencing and sulfite sequencing. Results the serum leptin level in GDM group was significantly higher than that in NGT group (P0.05) the expression of leptin mRNA and leptin protein in placenta of pregnant women with); GDM was higher than that in NGT group. The methylation and hydroxymethylation occurred in the promoter region of leptin gene in both groups, and the methylation level in GDM group was higher than that in NGT group (P0.05). Conclusion the level of leptin in pregnant women with gestational diabetes mellitus is higher than that with normal glucose tolerance. The dynamic change of leptin promoter methylation is associated with gestational diabetes mellitus (GDM).
【作者单位】：广州医科大学;深圳市第二人民医院产科;
【基金】：深圳市知识创新计划项目(编号:JCYJ20140414170821196)
【分类号】：R714.256

【相似文献】