缺氧下5-脂氧合酶在卵巢癌中的表达及意义
发布时间：2018-10-15 17:24
【摘要】：目的:检测缺氧环境下5-LOX在卵巢癌中的表达及与临床参数间的关系,探讨其在卵巢癌发生发展及治疗中的意义。方法:免疫组化检测56例卵巢癌组织中5-LOX与HIF-1α表达,并分析5-LOX表达与卵巢癌临床参数之间的关系。体外将人卵巢癌SKOV3细胞在缺氧环境下培养0、12、24h和48h,分别提取RNA和蛋白。qRT-PCR法和Western blot法分别检测5-LOX mRNA和蛋白表达;ELISA法检测缺氧环境下卵巢癌细胞5-LOX代谢产物5-HETE的变化。结果:5-LOX与HIF-1α表达具有明显的相关性,其表达水平与患者的淋巴结转移、远处转移和临床分期密切相关。缺氧条件下培养的SKOV3细胞的5-LOX在转录和蛋白水平均表达升高;缺氧条件下卵巢癌细胞培养上清的5-HETE较常氧条件下明显升高。结论:缺氧能促进卵巢癌5-LOX表达及增加代谢产物5-HETE的产生,提示5-LOX可作为治疗卵巢癌的潜在靶点,这为卵巢癌的发病机理研究和诊治提供了新的思路与方法。
[Abstract]:Objective: to detect the expression of 5-LOX in ovarian cancer under hypoxia and its relationship with clinical parameters, and to explore its significance in the development and treatment of ovarian cancer. Methods: the expression of 5-LOX and HIF-1 伪 was detected by immunohistochemistry in 56 cases of ovarian cancer, and the relationship between 5-LOX expression and clinical parameters of ovarian cancer was analyzed. Human ovarian cancer SKOV3 cells were cultured in hypoxic environment for 24 h and 48 h respectively to extract RNA and protein. QRT-PCR and Western blot methods were used to detect the expression of 5-LOX mRNA and protein respectively. ELISA method was used to detect the changes of 5-LOX metabolites 5-HETE in hypoxic environment. Results: the expression of 5-LOX and HIF-1 伪 was significantly correlated with lymph node metastasis, distant metastasis and clinical stage. The expression of 5-LOX in cultured SKOV3 cells under hypoxia was higher than that in normal oxygen, while the expression of 5-HETE in the supernatant of ovarian cancer cells cultured under hypoxia was significantly higher than that in normal oxygen. Conclusion: hypoxia can promote the expression of 5-LOX and increase the production of metabolite 5-HETE in ovarian cancer, suggesting that 5-LOX can be used as a potential target for the treatment of ovarian cancer, which provides a new idea and method for the study of pathogenesis and diagnosis and treatment of ovarian cancer.
【作者单位】： 山东大学齐鲁医院检验科;山东大学第二医院;
【分类号】：R737.31
，
本文编号：2273260
[Abstract]:Objective: to detect the expression of 5-LOX in ovarian cancer under hypoxia and its relationship with clinical parameters, and to explore its significance in the development and treatment of ovarian cancer. Methods: the expression of 5-LOX and HIF-1 伪 was detected by immunohistochemistry in 56 cases of ovarian cancer, and the relationship between 5-LOX expression and clinical parameters of ovarian cancer was analyzed. Human ovarian cancer SKOV3 cells were cultured in hypoxic environment for 24 h and 48 h respectively to extract RNA and protein. QRT-PCR and Western blot methods were used to detect the expression of 5-LOX mRNA and protein respectively. ELISA method was used to detect the changes of 5-LOX metabolites 5-HETE in hypoxic environment. Results: the expression of 5-LOX and HIF-1 伪 was significantly correlated with lymph node metastasis, distant metastasis and clinical stage. The expression of 5-LOX in cultured SKOV3 cells under hypoxia was higher than that in normal oxygen, while the expression of 5-HETE in the supernatant of ovarian cancer cells cultured under hypoxia was significantly higher than that in normal oxygen. Conclusion: hypoxia can promote the expression of 5-LOX and increase the production of metabolite 5-HETE in ovarian cancer, suggesting that 5-LOX can be used as a potential target for the treatment of ovarian cancer, which provides a new idea and method for the study of pathogenesis and diagnosis and treatment of ovarian cancer.
【作者单位】： 山东大学齐鲁医院检验科;山东大学第二医院;
【分类号】：R737.31
，
本文编号：2273260
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