卵丘颗粒细胞GOLPH3表达对单精子卵胞浆内注射助孕结局的影响
发布时间：2018-10-17 12:35
【摘要】：目的探讨卵丘颗粒细胞高尔基磷酸化蛋白-3(GOLPH3)表达对单精子卵胞浆内注射(ICSI)助孕结局的影响。方法收集2012年4月~2014年6月,因男性不育症行ICSI助孕的119例女性患者的卵丘颗粒细胞,采用细胞免疫化学,Western bloting方法检测颗粒细胞GOLPH3蛋白的表达,采用real-time荧光定量PCR方法检测GOLPH3基因表达。比较分析ICSI妊娠组与非妊娠组病例卵丘颗粒细胞GOLPH3表达率差异,并分析它们与ICSI实验室结局各项指标:穿卵泡数、获卵数、获卵率;受精数、受精率;卵裂数、卵裂率;优质胚胎数、囊胚形成数及囊胚冷冻数之间的相关关系。结果对119份卵丘颗粒细胞涂片进行免疫细胞化学分析GOLPH3的表达,结果发现GOLPH3主要表达在细胞质中,妊娠组与未妊娠组卵丘颗粒细胞GOLPH3表达率与表达强度组间差异均有统计学意义(P均0.05)。卵丘颗粒细胞GOLPH3表达率与ICSI实验室结局指标:穿卵泡数,Ⅲ级OCCs数,ICSI卵子数,受精数,卵裂数,平均优质胚胎数,平均囊胚数,优质囊胚数,平均冷冻胚胎数等呈显著正相关关系(P均0.01)。Real-Time PCR,Western blot检测妊娠组与未妊娠组颗粒细胞GOLPH3蛋白与基因表达组间差异也均有统计学意义(P均0.05)。结论 GOLPH3在卵丘颗粒细胞的表达,可能影响卵母细胞的成熟,卵子的发育能力,进而与ICSI助孕结局有关。
[Abstract]:Objective to investigate the effect of Golgi phosphorylated protein-3 (GOLPH3) expression in cumulus granulosa cells on the outcome of pregnancy assisted by intracytoplasmic injection of (ICSI). Methods from April 2012 to June 2014, the cumulus granulosa cells were collected from 119 female patients with ICSI assisted pregnancy due to male infertility. The expression of GOLPH3 protein in granulosa cells was detected by cytoimmunochemical, Western bloting assay. Real-time fluorescence quantitative PCR was used to detect the expression of GOLPH3 gene. To compare and analyze the difference of GOLPH3 expression rate of cumulus granulosa cells between ICSI pregnancy group and non-pregnancy group, and to analyze the indexes of ICSI laboratory outcome: the number of follicles pierced, the rate of obtaining eggs, the number of fertilization, the rate of fertilization, the number of cleavage, the rate of cleavage, the percentage of cleavage, the rate of cleavage. The correlation among the number of high quality embryos, the number of blastocysts and the number of frozen blastocysts. Results the expression of GOLPH3 was detected by immunocytochemistry in 119 smears of cumulus granulosa cells. The results showed that GOLPH3 was mainly expressed in cytoplasm. The GOLPH3 expression rate and expression intensity of cumulus granulosa cells in pregnancy group and non-pregnancy group were significantly different (P < 0. 05). The expression rate of GOLPH3 in cumulus granulosa cells and the laboratory outcome of ICSI: the number of follicles pierced, the number of grade 鈪，
本文编号：2276677
[Abstract]:Objective to investigate the effect of Golgi phosphorylated protein-3 (GOLPH3) expression in cumulus granulosa cells on the outcome of pregnancy assisted by intracytoplasmic injection of (ICSI). Methods from April 2012 to June 2014, the cumulus granulosa cells were collected from 119 female patients with ICSI assisted pregnancy due to male infertility. The expression of GOLPH3 protein in granulosa cells was detected by cytoimmunochemical, Western bloting assay. Real-time fluorescence quantitative PCR was used to detect the expression of GOLPH3 gene. To compare and analyze the difference of GOLPH3 expression rate of cumulus granulosa cells between ICSI pregnancy group and non-pregnancy group, and to analyze the indexes of ICSI laboratory outcome: the number of follicles pierced, the rate of obtaining eggs, the number of fertilization, the rate of fertilization, the number of cleavage, the rate of cleavage, the percentage of cleavage, the rate of cleavage. The correlation among the number of high quality embryos, the number of blastocysts and the number of frozen blastocysts. Results the expression of GOLPH3 was detected by immunocytochemistry in 119 smears of cumulus granulosa cells. The results showed that GOLPH3 was mainly expressed in cytoplasm. The GOLPH3 expression rate and expression intensity of cumulus granulosa cells in pregnancy group and non-pregnancy group were significantly different (P < 0. 05). The expression rate of GOLPH3 in cumulus granulosa cells and the laboratory outcome of ICSI: the number of follicles pierced, the number of grade 鈪，
本文编号：2276677
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