大鼠子宫宫腔粘连模型的建立及评价指标

发布时间：2018-10-21 16:46

【摘要】：子宫内膜损伤是导致宫腔粘连的最主要原因,建立有效的子宫内膜损伤动物模型是研究此类疾病发生、发展和治疗反应等不可或缺的支撑条件。通过宫腔注射95%的乙醇制造大鼠(Rattus norvegicus)子宫内膜损伤模型,检测胚胎植入数目来分析子宫内膜损伤对大鼠生育情况的影响,观察大鼠子宫内膜厚度、腺体数量和纤维化面积,分析乙醇处理对子宫内膜的影响;检测细胞角蛋白(CK-19)和波性蛋白(Vimentin)的表达水平,分析上皮细胞和间质细胞的增殖分化及子宫内膜损伤程度。结果显示,正常组大鼠子宫比损伤组更为平滑且有韧性,与正常组相比,损伤组大鼠生育率显著降低(P0.01),子宫内膜厚度变薄(P0.01)、腺体数量显著减少(P0.01),纤维化面积显著增大(P0.01),CK-19和Vimentin表达量显著下调。结果提示已成功建立大鼠子宫内膜损伤动物模型。
[Abstract]:Endometrial injury is the main cause of uterine cavity adhesion. Establishing an effective animal model of endometrial injury is an indispensable supporting condition to study the occurrence, development and therapeutic response of this kind of disease. The (Rattus norvegicus) endometrial injury model of rats was made by intrauterine injection of 95% ethanol. The number of embryo implantation was measured to analyze the effect of endometrial injury on the fertility of rats. The thickness of endometrium, the number of glands and the area of fibrosis were observed. The effects of ethanol treatment on endometrium were analyzed, and the expression levels of cytokeratin (CK-19) and wave protein (Vimentin) were detected, and the proliferation and differentiation of epithelial cells and interstitial cells were analyzed as well as the degree of endometrial injury. The results showed that the uterus of the normal group was smoother and more resilient than that of the injured group. In the injured group, the fertility rate decreased significantly (P0.01), the thickness of endometrium became thinner (P0.01), the number of glands decreased significantly (P0.01), the area of fibrosis increased significantly (P0.01), and the expression of CK-19 and Vimentin decreased significantly. The results suggest that the rat model of endometrial injury has been successfully established.
【作者单位】：国家卫生计生委科学技术研究所遗传优生中心;北京协和医学院研究生院;
【基金】：国家自然科学基金项目(No.81370720) 中央级公益性科研院所基本科研业务费专项(No.015GJZ01)
【分类号】：R711;R-332

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