PEDF在植入型凶险性前置胎盘中的表达及其对血管内皮细胞迁移、侵袭的影响

发布时间：2018-10-22 06:55

【摘要】：目的探讨色素上皮衍生因子(pigment epithelium-derived factor,PEDF)在植入型凶险性前置胎盘中的表达及其对脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)迁移、侵袭能力的影响。方法选择2014年7月~2015年7月福建省立医院剖宫产分娩的植入型凶险性前置胎盘(PA)组14例,前置胎盘(PP)组16例。采用免疫组化法检测两组胎盘的PEDF和VEGF的定位表达及微血管密度(microvascular vascular density,MVD);用RT-PCR及Western blot检测胎盘的PEDF和VEGF的mRNA和蛋白质表达;用ELISA检测外周血清中PEDF和VEGF水平。培养脐静脉内皮细胞,设阴性对照组和不同浓度的PDEF干预组,用平板划痕和Transwell侵袭实验检测HUVEC细胞的迁移和侵袭能力,用ELISA检测培养上清液中VEGF水平。结果 PA组与PP组相比,胎盘PEDF的mRNA和蛋白表达明显降低而VEGF则明显升高,差异均有统计学意义(均P0.05)。胎盘PEDF表达与胎盘VEGF、MVD及胎盘植入深度呈负相关关系(均P0.05),与外周血清PEDF水平呈正相关关系(P0.05)。各PEDF干预组的迁移和侵袭细胞数显著低于阴性对照组(均P0.05)。随干预浓度增加,HUVEC细胞的迁移和侵袭能力降低(均P0.05)。结论 PEDF可以反映植入型凶险性前置胎盘的异常血管密度和植入程度,抑制血管内皮细胞的迁移和侵袭能力,可作为影像学检查的重要补充诊断方法和潜在的治疗切入点。
[Abstract]:Objective to investigate the expression of pigment epithelium-derived factor (pigment epithelium-derived factor,PEDF) in placenta accreta and its effect on the migration and invasion of umbilical vein endothelial cells (human umbilical vein endothelial cells,HUVEC). Methods from July 2014 to July 2015, 14 cases of placenta previa accreta (PA) group and 16 cases of (PP) group of placenta previa were selected from Fujian Provincial Hospital for cesarean section delivery. The localization expression of PEDF and VEGF and microvessel density (microvascular vascular density,MVD) in placenta were detected by immunohistochemical method, mRNA and protein expression of PEDF and VEGF in placenta were detected by RT-PCR and Western blot, and PEDF and VEGF levels in peripheral serum were detected by ELISA. Umbilical vein endothelial cells were cultured, negative control group and different concentration of PDEF intervention group were used to detect the migration and invasion ability of HUVEC cells by plate scratch and Transwell invasion test, and the level of VEGF in culture supernatant was detected by ELISA. Results the expression of mRNA and protein in placental PEDF in PA group was significantly lower than that in PP group, while the expression of VEGF in placental PEDF was significantly higher than that in PP group (P0.05). The expression of placental PEDF was negatively correlated with placental VEGF,MVD and the depth of placenta accreta (P0.05), and positively correlated with the level of PEDF in peripheral blood (P0.05). The number of migration and invasion cells in each PEDF intervention group was significantly lower than that in negative control group (P0.05). With the increase of intervention concentration, the migration and invasion ability of HUVEC cells decreased (P0.05). Conclusion PEDF can reflect the abnormal vascular density and the degree of implantation of placenta previa and inhibit the migration and invasion of vascular endothelial cells. It can be used as an important supplementary diagnostic method and a potential cut-in point for imaging examination.
【作者单位】：福建医科大学省立临床学院妇产科;福州大学药物生物技术与工程研究所;福建医科大学省立临床学院病理科;
【基金】：福建省立医院优秀青年医师项目(No.2014YNQN13) 福建省自然科学基金计划项目(No.13171069)
【分类号】：R714.2

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