宫颈上皮脱落细胞mybl2 mRNA表达水平及意义

发布时间：2018-10-24 08:06

【摘要】：目的探讨宫颈上皮脱落细胞中mybl2 mRNA表达水平与宫颈病变程度、人乳头瘤病毒(HPV)感染三者间的关系。方法收集宫颈上皮脱落细胞共232例,根据液基薄层细胞检测结果对标本进行分组,其中不能明确意义的非典型鳞状细胞(ASC-US)组68例,低度鳞状上皮内病变(LSIL)组98例,高度鳞状上皮内病变(HSIL)组42例,宫颈癌(SCC)组24例。Trizol法提取细胞总RNA,RT-PCR法检测mybl2 mRNA的表达水平,分析细胞mybl2 mRNA表达水平与宫颈病变程度的关系;同时收集被检者HPV检测结果资料,分析细胞mybl2mRNA表达水平与HPV感染的关系。结果 (1)232例宫颈上皮脱落细胞均检测到mybl2 mRNA表达,不同病变程度的宫颈上皮脱落细胞中mybl2 mRNA表达水平差异有统计学意义(F=68.491,P0.01),其表达水平与宫颈病变程度呈正相关,以SCC组脱落细胞的mybl2 mRNA表达水平最高。(2)宫颈脱落细胞中mybl2 mRNA表达水平与HPV感染有关,差异有统计学意义(F=12.15,P0.01),以HPV16+和(或)HPV18+的脱落细胞表达量最高。结论宫颈上皮脱落细胞中mybl2 mRNA表达水平与宫颈病变程度呈正相关,且与HPV感染有关,可能与宫颈癌的疾病进展有关,有望作为宫颈病变进展的新的生物标志物。
[Abstract]:Objective to investigate the relationship between the expression of mybl2 mRNA and the degree of cervical lesions and the infection of human papillomavirus (HPV) in exfoliated cells of cervical epithelium. Methods 232 cases of cervical epithelial exfoliated cells were collected and divided into two groups according to the results of liquid-based thin layer cell detection. 68 cases of atypical squamous cell (ASC-US) group and 98 cases of low grade squamous intraepithelial lesion group (LSIL) group) were divided into two groups: ASC-US group (n = 68) and (LSIL) group (n = 98). There were 42 cases of high squamous intraepithelial lesions (HSIL) and 24 cases of cervical cancer (SCC). The expression level of mybl2 mRNA was detected by Trizol total RNA,RT-PCR method, and the relationship between the expression of mybl2 mRNA and the degree of cervical lesions was analyzed, and the results of HPV detection were collected. The relationship between mybl2mRNA expression and HPV infection was analyzed. Results (1) the expression of mybl2 mRNA was detected in the exfoliated cells of cervical epithelium in 232 cases. There was significant difference in the expression of mybl2 mRNA in the exfoliated cells of cervical epithelium with different degree of lesion (FF68.491p0.01), and the expression level was positively correlated with the degree of cervical lesions. The expression level of mybl2 mRNA in exfoliated cells of SCC group was the highest. (2) the expression of mybl2 mRNA in exfoliated cells of cervix uteri was related to HPV infection, and the difference was statistically significant (FF12.15, P0.01), and the expression of HPV16 and / or HPV18 in exfoliated cells was the highest. Conclusion the expression of mybl2 mRNA in the exfoliated cells of cervical epithelium is positively correlated with the degree of cervical lesions and HPV infection. It may be related to the progression of cervical cancer and may be a new biomarker for the progression of cervical lesions.
【作者单位】：清远市人民医院检验科;清远市人民医院妇产科;
【基金】：清远市科技计划项目(编号:2013B042)
【分类号】：R737.33

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