精子优化处理后孵育时间与夫精宫腔内人工授精临床妊娠率的相关性研究

发布时间：2018-11-03 11:55

【摘要】：目的:研究精子优化处理后孵育时间对夫精宫腔内人工授精(AIH-IUI)临床妊娠率的影响。方法:相同促排卵方案的191个AIH-IUI周期,男方精液经密度梯度离心优化处理后,将孵育时间分为0~19、20~39、40~59、60~80 min 4组;根据优化处理后前向运动精子总数(TPMC)分为(0~9)×106、(10~20)×106、(21~30)×106、30×1064组,分析孵育时间和优化处理后TPMC与临床妊娠率的关系,并对患者年龄、不孕年限、孵育时间、优化处理后TPMC与临床妊娠率进行Logistic多因素回归分析。结果:不同孵育时间临床妊娠率分别为12.7%、18.3%、11.4%、9.1%,孵育20~39 min组显著高于其他3组(P均0.05);优化处理后4组TPMC临床妊娠率分别为0%、16.7%、11.4%、8.3%,(10~20)×106组显著高于其他3组(P均0.05);Logistic多因素回归结果显示,女方年龄的增加会导致临床妊娠率显著下降(OR 0.89,95%CI 0.83~0.94),而孵育时间20~39 min(OR 2.11,95%CI 1.34~3.13)和处理后TPMC(10~20)×106(OR 2.06,95%CI 1.32~3.46)均可显著增加临床妊娠率。结论:精子优化处理后的孵育时间是AIH-IUI临床妊娠率的重要影响因素。
[Abstract]:Aim: to study the effect of incubation time on the clinical pregnancy rate of intrauterine insemination (AIH-IUI) of spermatozoa. Methods: during 191 AIH-IUI cycles of the same ovulation promotion regimen, the male semen was optimized by density gradient centrifugation, and the incubation time was divided into 4 groups: 0 ~ (19) ~ (19) ~ (20) ~ 20 ~ (9) ~ 40 ~ (9) ~ (9) ~ (60) ~ (60) min; According to the total number of motile spermatozoa after optimized treatment, (TPMC) was divided into (0 ~ 9) 脳 10 ~ (6) 脳 10 ~ (6), (10 ~ 20) 脳 10 ~ (6), (21 ~ (30) 脳 10 ~ (6) 脳 1064 groups. The relationship between incubation time and clinical pregnancy rate was analyzed. Logistic multivariate regression analysis was performed between TPMC and clinical pregnancy rate after optimized treatment. Results: the clinical pregnancy rate of different incubation time was 12.70.18.30.The clinical pregnancy rate of the 2039 min group was significantly higher than that of the other three groups (P 0.05). After optimized treatment, the clinical pregnancy rates of TPMC in the four groups were 0 and 16.7and 11.4and 8.3respectively, (10 ~ 20) 脳 10 ~ (6) group were significantly higher than those in the other three groups (P < 0.05). The results of Logistic multivariate regression showed that the increase of age of the woman resulted in a significant decrease in the clinical pregnancy rate (OR 0.89 / 95 CI 0.83 卤0.94). The incubation time of 20 ~ 39 min (OR 2.1195 CI 1.34 ~ 3.13) and TPMC _ (10 ~ 20) 脳 10 ~ (6) (OR 2.06 ~ 95 CI 1.32 ~ 3.46) significantly increased the clinical pregnancy rate. Conclusion: the incubation time after optimized sperm treatment is an important factor in the clinical pregnancy rate of AIH-IUI.
【作者单位】：广东省妇幼保健院生殖健康与不孕症科;
【分类号】：R714.8

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