上皮性卵巢癌组织中miR-21-5p、miR-29-3p、miR-139-5p表达变化及意义

发布时间：2018-11-04 10:04

【摘要】：目的观察上皮性卵巢癌(EOC)组织中miR-21-5p、miR-29-3p和miR-139-5p的表达变化,并探讨其临床意义。方法收集卵巢组织标本182例,其中正常卵巢组织56例、良性上皮性肿瘤(BEOT)42例、EOC 84例。采用实时荧光定量PCR技术测定组织标本中的miR-21-5p、miR-29-3p和miR-139-5p,并分析其与EOC患者临床病理特征的关系。结果与正常卵巢组织、BEOT组织相比,EOC组织中miR-21-5p和miR-29-3p相对表达量增加,miR-139-5p相对表达量降低(P均0.05);正常卵巢组织与BEOT组织比较,差异无统计学意义。与中高度分化、Ⅰ~Ⅱ期和无淋巴结转移的EOC组织相比,低分化、Ⅲ~Ⅳ期和有淋巴结转移的EOC组织miR-21-5p和miR-29-3p高表达者多,miR-139-5p高表达者少(P均0.05)。结论 EOC组织中miR-21-5p和miR-29-3p表达上调并可能发挥促癌作用,miR-139-5p表达下调并可能具有一定抑癌作用,三者均可作为EOC的预后指标或者治疗靶标。
[Abstract]:Objective to investigate the expression of miR-21-5p,miR-29-3p and miR-139-5p in epithelial ovarian carcinoma (EOC) and its clinical significance. Methods A total of 182 cases of ovarian tissues were collected, including 56 cases of normal ovarian tissues, 42 cases of benign epithelial tumors (BEOT) and 84 cases of EOC. The miR-21-5p,miR-29-3p and miR-139-5p, in tissue samples were measured by real-time fluorescence quantitative PCR and the relationship between miR-21-5p,miR-29-3p and clinicopathological features of EOC patients was analyzed. Results compared with normal ovarian tissues and BEOT tissues, the relative expression of miR-21-5p and miR-29-3p in EOC increased and the relative expression of miR-139-5p decreased (P0. 05). There was no significant difference between normal ovarian tissues and BEOT tissues. The expression of miR-21-5p and miR-29-3p in EOC tissues with lymph node metastasis and stage 鈪，

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