宫颈鳞癌组织中HPV16 E6与人端粒保护蛋白1的检测及关系的研究

发布时间：2018-11-04 13:00

【摘要】：目的:研究宫颈鳞癌、宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)及正常宫颈组织中人乳头瘤病毒(Human papilloma virus,HPV)16 E6蛋白及人端粒保护蛋白1(Human protection of telomeres 1,POT1)的表达情况及关系。方法:收集山西医科大学附属第一医院2013年5月至2016年5月期间10例正常宫颈组织(无HPV感染)、30例宫颈上皮内瘤样病变组织(其中CINI5例、CINII10例、CINIII15例)、40例宫颈鳞癌组织。采用免疫组织化学SP方法分别检测以上宫颈组织中HPV16 E6蛋白和POT1蛋白的表达情况及其相关性。结果:1.POT1蛋白在正常宫颈组、CIN组、宫颈鳞癌组织的表达呈现出逐渐下降的趋势,三组间两两比较差异有统计学意义(χ2=19.662,P0.05)。HPV16 E6蛋白在正常宫颈组、CIN组、宫颈鳞癌组织的表达呈现出逐渐上升的趋势,差异有统计学意义(χ2=26.362,P0.05)。2.在不同年龄、临床分期、肿瘤大小、组织分化程度的宫颈鳞癌组织中HPV16 E6蛋白和POT1蛋白表达差异无统计学意义(P0.05),宫颈鳞癌组织中POT1在无淋巴转移的表达高于有淋巴转移者,差异有统计学意义(χ2=7.677,P=0.0270.05),HPV16E6蛋白的表达与淋巴转移有关,差异有统计学意义(χ2=4.912,P0.05)。3.运用Spearman相关性分析显示HPV16 E6蛋白和POT1蛋白阳性表达呈负相关(rs=-0.565,P0.01)。结论:宫颈鳞癌组织中HPV16 E6蛋白与POT1蛋白阳性表达呈负相关,其机制可能是通过HPV16 E6蛋白的表达抑制了POT1蛋白的表达,进而促进宫颈鳞癌的发生、发展。
[Abstract]:Objective: to study the expression of human papillomavirus (Human papilloma virus,HPV) 16 E6 protein and human telomere protection protein 1 (Human protection of telomeres 1 in cervical squamous cell carcinoma, cervical intraepithelial neoplasia (Cervical intraepithelial neoplasia,CIN) and normal cervical tissues. The expression and relationship of POT1. Methods: from May 2013 to May 2016, 10 cases of normal cervical tissues (without HPV infection) and 30 cases of cervical intraepithelial neoplasia (CINI5, CINII10, CINIII15) were collected from the first affiliated Hospital of Shanxi Medical University. 40 cases of cervical squamous cell carcinoma. Immunohistochemical SP method was used to detect the expression of HPV16 E6 protein and POT1 protein in cervical tissues. Results: the expression of 1.POT1 protein in normal cervix group, CIN group and cervical squamous cell carcinoma showed a decreasing trend, and there was a significant difference among the three groups (蠂 2 + 19.662P 0.05). HPV16 E6 protein was expressed in normal cervix group and CIN group. The expression of squamous cell carcinoma of the cervix showed an increasing trend, and the difference was statistically significant (蠂 ~ 2 ~ 2 ~ (26.362) P _ (0.05). There was no significant difference in the expression of HPV16 E6 protein and POT1 protein in cervical squamous cell carcinoma tissues with different age, clinical stage, tumor size and tissue differentiation (P0.05). The expression of POT1 in cervical squamous cell carcinoma was significantly higher than that in patients without lymphatic metastasis (蠂 27.677Pu 0.0270.05). The expression of HPV16E6 protein was related to lymphatic metastasis (蠂 2 + 4.912). P0.05) The positive expression of HPV16 E6 protein and POT1 protein was negatively correlated (rs=-0.565,P0.01) by Spearman correlation analysis. Conclusion: there is a negative correlation between HPV16 E6 protein and POT1 protein expression in squamous cell carcinoma of cervix. The mechanism may be that HPV16 E6 protein inhibits the expression of POT1 protein and promotes the occurrence and development of cervical squamous cell carcinoma.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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