线粒体解耦联蛋白2在宫颈癌中的作用及生物信息学分析

发布时间：2018-11-05 12:25

【摘要】：目的:研究线粒体解耦联蛋白2(UCP2)对宫颈鳞癌增殖、凋亡、转移的影响。方法:通过质粒转染技术,下调宫颈癌细胞系中UCP2的表达,分别用MTT、流式细胞术、Transwell小室检测宫颈鳞癌细胞增殖、凋亡、侵袭、转移能力的变化;利用生物信息学的方法对UCP2蛋白进行生物信息学分析,并分析与之相互作用的蛋白。结果:(1)q RT-PCR和WB证实质粒转染后UCP2在宫颈癌细胞株中的表达较对照组明显降低。(2)MTT、凋亡、Transwell小室实验结果显示,下调UCP2后Si Ha细胞的增殖能力明显减弱,凋亡率增加,转移能力减弱(P0.05)。(3)生物信息学分析显示UCP2蛋白定位于线粒体,可能存在多个磷酸化位点,且与多种蛋白相互作用,发挥生物学效应。结论:UCP2可能在宫颈癌的发生和发展中发挥重要作用,有望成为宫颈癌的早期诊断标志物和治疗的新靶点。
[Abstract]:Aim: to study the effects of mitochondrial decoupling protein 2 (UCP2) on proliferation, apoptosis and metastasis of cervical squamous cell carcinoma. Methods: the expression of UCP2 in cervical carcinoma cell line was down-regulated by plasmid transfection technique. The proliferation, apoptosis, invasion and metastasis of cervical squamous cell carcinoma cells were detected by MTT, flow cytometry and Transwell chamber. UCP2 protein was analyzed by bioinformatics and the proteins interacting with it were analyzed. Results: (1) Q RT-PCR and WB confirmed that the expression of UCP2 in cervical cancer cell lines after transfection was significantly lower than that in the control group. (2) MTT, apoptosis. The results of Transwell chamber experiment showed that the proliferation ability of Si Ha cells decreased significantly after down-regulation of UCP2. The bioinformatics analysis showed that the UCP2 protein was located in mitochondria with multiple phosphorylation sites and interacted with many proteins to play a biological effect. Conclusion: UCP2 may play an important role in the occurrence and development of cervical cancer and may be a new target for early diagnosis and treatment of cervical cancer.
【作者单位】：川北医学院附属医院妇产科;
【分类号】：R737.33

【参考文献】