胎盘合体滋养细胞微粒注射对孕鼠肾功能和病理形态损伤的研究

发布时间：2018-11-08 07:22

【摘要】：目的观察经孕鼠尾静脉注射合体滋养细胞微粒(syncytiotrophoblast microparticles STBM)后,小鼠肾功能及肾脏病理改变。方法收集孕18 d C57小鼠的胎盘,制备小鼠合体滋养细胞微粒,利用电镜观察C57小鼠STBM的形态结构,BCA法测定C57小鼠STBM的蛋白水平;将C57孕鼠分为STBM组、对照组和PBS组,STBM组:自C57小鼠孕8 d起,每天以蛋白浓度为0.15 mg/m L的STBM(0.2 m L)经鼠尾静脉回输至小鼠体内,至孕18 d时收集小鼠24 h尿液测定尿微量蛋白(M-TP)、尿肌酐(U-Crea)、尿蛋白肌酐比(M-TP/Cr)、尿酸(U-Uric)、24 h尿蛋白(24 h U-TP),采集全血测定血清尿素氮(BUN)、肌酐(Scr)、胱抑素C(Cys-C),取肾脏标本切片后行HE染色观察肾组织结构变化;对照组:正常孕18 d小鼠收集尿液、采集全血、取肾脏进行检测;PBS组:自孕8 d起每天经鼠尾静脉注射PBS 0.2 m L,余处理同STBM组。结果 STBM组孕鼠尿液M-TP、M-TP/Cr、24 h U-TP明显高于对照组和PBS组(P0.01),与对照组比较,PBS组U-Uric未见明显变化(P0.05),而STBM组U-Uric显著降低(P0.01);STBM组血清BUN、Scr、Cys-C含量显著升高(P0.01);STBM组肾脏HE染色可见肾小球内皮细胞增生、肿胀、变形,肾小管云雾状变性。结论类子痫前期模型鼠有肾损伤,STBM可能在子痫前期肾损害中起重要作用。
[Abstract]:Objective to observe the renal function and renal pathological changes of pregnant mice after intravenous injection of syncytiotrophoblast microparticle (syncytiotrophoblast microparticles STBM). Methods the placenta of C57 mice was collected on the 18th day of pregnancy and the syncytiotrophoblast particles were prepared. The morphology and structure of STBM in C57 mice were observed by electron microscope and the protein level of STBM in C57 mice was measured by BCA method. C57 pregnant mice were divided into STBM group, control group and PBS group, STBM group: from the 8th day of gestation, the mice were infused with 0.15 mg/m / L STBM (0.2 m L) via tail vein. Urine trace protein (M-TP), urinary creatinine (U-Crea), urinary creatinine ratio (M-TP/Cr), uric acid (U-Uric), 24 h urine protein (24 h U-TP) were collected at the 18th day of gestation. Serum urea nitrogen (BUN),) creatinine (Scr),) cystatin C (Cys-C) was measured in whole blood. Renal tissue structure was observed by HE staining. In the control group, urine was collected, whole blood was collected and kidney was detected in the normal 18 day pregnant mice, while in the PBS group, PBS 0.2 mL was injected through the tail vein of the mice every day since the 8th day of gestation, and the remaining treatment was the same as that in the STBM group. Results the 24 h U-TP in urine of pregnant rats in STBM group was significantly higher than that in control group and PBS group (P0.01). There was no significant change in U-Uric in PBS group compared with control group (P0.05). In STBM group, U-Uric was significantly decreased (P0.01). The content of serum BUN,Scr,Cys-C in STBM group was significantly higher (P0.01); STBM group, renal HE staining showed glomerular endothelial cell proliferation, swelling, deformation, tubular cloud degeneration. Conclusion STBM may play an important role in preeclampsia induced renal injury in preeclampsia rats.
【作者单位】：第三军医大学大坪医院野战外科研究所妇产科中心;四川省人民医院妇产科;
【基金】：国家自然科学基金面上项目(31470886,81270008) 重庆市科委应用开发课题(CS+C20Byykf A0213)~~
【分类号】：R-332;R714.244

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