小鼠骨髓间充质干细胞在子宫内膜损伤小鼠子宫内膜中的定位

发布时间：2018-11-09 20:03

【摘要】：目的:观察骨髓间充质干细胞(MSCs)在子宫内膜损伤小鼠子宫内膜中的定位,初步探讨MSCs移植用于小鼠子宫内膜损伤后修复的可行性。方法:采用热损伤的方法建立小鼠子宫内膜损伤模型,随机分为MSCs组和对照组,每组15只。将体外分离培养的雄性小鼠MSCs经尾静脉联合宫腔内局部注射植入MSCs组小鼠体内,对照组注射等体积的PBS。通过对移植后子宫内膜组织的SRY DNA原位杂交来定位源自雄性供体的细胞。结果:MSCs组小鼠损伤侧子宫内膜组织中有SRY阳性细胞的定植,对照组小鼠则未见SRY阳性细胞。结论:MSCs可定植于子宫内膜损伤小鼠的子宫内膜组织。
[Abstract]:Aim: to investigate the localization of bone marrow mesenchymal stem cell (MSCs) in endometrium of mice with endometrial injury and to explore the feasibility of MSCs transplantation in repairing endometrium. Methods: the model of endometrium injury in mice was established by heat injury. The model was randomly divided into MSCs group and control group with 15 rats in each group. MSCs of male mice isolated and cultured in vitro was injected into MSCs group via tail vein and intrauterine injection, and PBS. of equal volume was injected into control group. SRY DNA in situ hybridization of transplanted endometrial tissue was used to locate the cells derived from male donors. Results: there were SRY positive cells in the injured endometrium of MSCs group and no SRY positive cells in control group. Conclusion: MSCs can be applied to endometrium tissue of injured mice.
【作者单位】：河南省直第三人民医院妇产科;郑州大学第三附属医院妇产科;
【基金】：河南省计划生育科学技术研究计划项目2012070023 郑州市科技局科技计划基金资助项目112PPTSF318-9
【分类号】：R711.74

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