来曲唑灌胃后EM大鼠病灶体积及COX-2 mRNA、survivin蛋白表达变化
发布时间：2018-11-13 13:10
【摘要】：目的观察来曲唑灌胃后子宫内膜异位症(EM)大鼠病灶体积及病灶组织中环氧合酶-2(COX-2)mRNA、生存素(survivin)蛋白表达变化。方法性成熟未交配过的健康雌性Wistar大鼠48只,采用子宫内膜自体移植法建立EM模型。选取40只造模成功的大鼠,随机分为A、B、C、D组各10只。A、B、C组分别以来曲唑(0.26mg/kg)灌胃1、2、3周,D组以生理盐水灌胃3周。以游标卡尺测量给药前后各组异位病灶体积。采用RT-PCR法检测各组大鼠病灶组织中的COX-2 mRNA;免疫组化SP法检测survivin蛋白。结果 A、B、C组给药后异位病灶体积缩小,与给药前相比,P均0.05;D组给药前后差异无统计学意义。A、B、C组病灶组织中COX-2 mRNA、survivin蛋白表达与D组相比,P均0.05;A组与B、C组相比,P均0.05;B、C组差异无统计学意义。结论来曲唑可缩小EM大鼠异位病灶体积,并下调病灶组织中COX-2 mRNA、survivin蛋白的表达,这可能是来曲唑治疗EM的机制之一。
[Abstract]:Objective to observe the changes of focus volume and (survivin) protein expression of cyclooxygenase-2 (COX-2) mRNA, survivin in (EM) rats with endometriosis after oral administration of letrozole. Methods 48 healthy female Wistar rats with unmated sexual maturation were used to establish EM model by autologous transplantation of endometrium. Forty successful rats were randomly divided into two groups: group A (n = 10): 0.26mg/kg (n = 10) and group D (n = 3) were treated with normal saline for 3 weeks, respectively (n = 10), group A (n = 10) were given tritrazole (0.26mg/kg) for 3 weeks, and group D (n = 3) were given normal saline for 3 weeks. The volume of ectopic lesions in each group was measured by Vernier caliper before and after administration. The COX-2 mRNA; immunohistochemical SP method was used to detect the survivin protein in the focus tissue of rats by RT-PCR method. Results the volume of ectopic foci in group A was reduced after administration, compared with that before administration (P < 0.05). There was no significant difference in the expression of COX-2 mRNA,survivin protein between group D and group D before and after administration, and there was no significant difference in the expression of COX-2 mRNA,survivin protein between group A and group D, between group A and group B, and between group A and group B, respectively. Conclusion Letrozole can reduce the volume of ectopic focus and down-regulate the expression of COX-2 mRNA,survivin protein in EM rats, which may be one of the mechanisms of letrozole in the treatment of EM.
【作者单位】： 潍坊医学院附属医院;
【分类号】：R711.71
[Abstract]:Objective to observe the changes of focus volume and (survivin) protein expression of cyclooxygenase-2 (COX-2) mRNA, survivin in (EM) rats with endometriosis after oral administration of letrozole. Methods 48 healthy female Wistar rats with unmated sexual maturation were used to establish EM model by autologous transplantation of endometrium. Forty successful rats were randomly divided into two groups: group A (n = 10): 0.26mg/kg (n = 10) and group D (n = 3) were treated with normal saline for 3 weeks, respectively (n = 10), group A (n = 10) were given tritrazole (0.26mg/kg) for 3 weeks, and group D (n = 3) were given normal saline for 3 weeks. The volume of ectopic lesions in each group was measured by Vernier caliper before and after administration. The COX-2 mRNA; immunohistochemical SP method was used to detect the survivin protein in the focus tissue of rats by RT-PCR method. Results the volume of ectopic foci in group A was reduced after administration, compared with that before administration (P < 0.05). There was no significant difference in the expression of COX-2 mRNA,survivin protein between group D and group D before and after administration, and there was no significant difference in the expression of COX-2 mRNA,survivin protein between group A and group D, between group A and group B, and between group A and group B, respectively. Conclusion Letrozole can reduce the volume of ectopic focus and down-regulate the expression of COX-2 mRNA,survivin protein in EM rats, which may be one of the mechanisms of letrozole in the treatment of EM.
【作者单位】： 潍坊医学院附属医院;
【分类号】：R711.71
【参考文献】
相关期刊论文 前3条
1 夏晓梦;郭李璐;苏津平;方小玲;;来曲唑对大鼠子宫内膜异位症的作用及对异位内膜细胞凋亡的影响[J];中南大学学报(医学版);2013年01期
2 郎景和,冷金花,赵栋,钟森;第八届国际子宫内膜异位症学术会议纪要[J];中华妇产科杂志;2002年10期
3 秦江霞;岳玉焕;杨爱银;;COX-2、VEGF以及MVD在子宫内膜异位症中的表达及意义[J];中国优生与遗传杂志;2014年06期
【共引文献】
相关期刊论文 前10条
1 马宏,林元,陈光荣,吴荔香;肌纤维母细胞与盆腔子宫内膜异位症的关系[J];福建医科大学学报;2005年04期
2 徐艳明;;子宫内膜异位症术后复发72例临床分析[J];中国妇产科临床杂志;2007年05期
3 董U，
本文编号：2329204
本文链接：https://www.wllwen.com/yixuelunwen/fuchankeerkelunwen/2329204.html
最近更新
教材专著
