凋亡、坏死合体滋养细胞微粒对内皮细胞增殖与凋亡的影响

发布时间：2018-11-20 13:39

【摘要】：目的:探讨凋亡、坏死合体滋养细胞微粒对人脐静脉内皮细胞(HUVECs)增殖、凋亡的影响。方法:培养原代HUVECs,物理方法诱导滋养细胞凋亡、坏死。采用三步超速离心法制备凋亡合体滋养细胞微粒(a STBM)、坏死合体滋养细胞微粒(n STBM)(实验组),同时制备RBC膜(对照组),并检测胎盘碱性磷酸酶(PLAP)含量。将不同浓度(20、80、320μg/ml)a STBM、n STBM和RBC膜分别与HUVECs共同孵育4、16、32h。采用MTT法检测细胞增殖抑制率,采用流式细胞仪AV-PI双标记观察细胞凋亡情况。结果:(1)80μg/ml a STBM组作用4、16、32h时的细胞增殖抑制率分别为29.19±0.47、44.73±0.69、54.01±1.61;80μg/ml n STBM组作用4、16、32h的细胞增殖抑制率分别为57.15±0.70、64.35±0.89、71.67±0.63。a STBM、n STBM均能明显抑制HUVEC增殖,抑制作用呈浓度时间依赖性;n STBM对HUVECs的增殖抑制性明显高于a STBM,差异有统计学意义(P0.001)。RBC膜处理组对HUVECs增殖无明显影响,与空白组比较差异无统计学意义(P0.05)。(2)80μg/ml a STBM组的细胞早期凋亡率、晚期凋亡-坏死率分别为29.05%和7.46%。80μg/ml n STBM组分别为15.93%和30.31%。RBC膜处理组对HUVECs的凋亡无明显影响,与空白对照组相比,差异无统计学意义(P0.05)。a STBM、n STBM组均能引起HUVECs凋亡,n STBM作用HUVECs的晚期凋亡率高于a STBM组,两者有统计学意义(P0.001)。a STBM主要诱导HUVECs早期凋亡。结论:a STBM、n STBM对内皮细胞有抑制细胞增殖作用,a STBM主要以促进内皮细胞早期凋亡为主,而n STBM以内皮细胞晚期凋亡为主,n STBM对内皮细胞造成的损害强于a STBM。
[Abstract]:Aim: to investigate the effects of apoptosis and necrotic syncytiotrophoblast microparticles on (HUVECs) proliferation and apoptosis in human umbilical vein endothelial cells (HUVEC). Methods: primary HUVECs, was used to induce the apoptosis and necrosis of trophoblast cells. Apoptosis syncytiotrophoblast microparticles (a STBM),) and syncytiotrophoblastic microparticles (n STBM) () were prepared by three-step ultracentrifugation. Meanwhile, RBC membrane (control group) was prepared, and the content of alkaline phosphatase (PLAP) in placenta was measured. At different concentrations (20 ~ 80320 渭 g/ml) a STBM,n STBM and RBC membrane were incubated with HUVECs for 4 ~ 16 ~ 32 h. The inhibition rate of cell proliferation was detected by MTT assay and apoptosis was observed by flow cytometry AV-PI double labeling. Results: (1) the inhibitory rates of cell proliferation in 80 渭 g/ml a STBM group were 29.19 卤0.47U 44.73 卤0.69U 54.01 卤1.61respectively. The inhibitory rates of cell proliferation in 80 渭 g/ml n STBM group were 57.15 卤0.7054.35 卤0.89U 71.67 卤0.63.a STBM,n STBM, respectively, in a dose-dependent manner. The inhibitory effect of n STBM on HUVECs proliferation was significantly higher than that on a STBM, group (P0. 001). RBC membrane treatment group had no significant effect on HUVECs proliferation. There was no significant difference between the control group and the control group (P0.05). (2). The rate of early apoptosis in 80 渭 g/ml a STBM group was higher than that in the control group. The late apoptosis-necrosis rate was 29.05% and 7.46.80 渭 g/ml n STBM group was 15.93% and 30.31%.RBC membrane treatment group had no significant effect on HUVECs apoptosis, compared with the blank control group. There was no significant difference (P0.05) the late apoptosis rate of HUVECs induced by, n STBM in). A STBM,n STBM group was higher than that in a STBM group (P0.001). A STBM mainly induced early apoptosis of HUVECs. Conclusion: a STBM,n STBM can inhibit the proliferation of endothelial cells., a STBM mainly promotes the early apoptosis of endothelial cells, while n STBM has more damage to endothelial cells than a STBM..
【作者单位】：昆山市第一人民医院妇产科;上海交通大学附属第六人民医院妇产科;
【基金】：上海市第六人民医院2010年度院级科研基金资助项目
【分类号】：R714.244

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