SMG-1基因对缺氧卵巢癌细胞SKOV3增殖、凋亡的影响及其机制

发布时间：2018-11-23 20:44

【摘要】：目的观察缺氧状态下卵巢癌SKOV3细胞中生殖器形成抑制基因1(SMG-1)的表达变化,及沉默SMG-1对SKOV3细胞增殖、凋亡的影响,并探讨其机制。方法培养卵巢癌SKOV3细胞,分为常氧组和缺氧组,其中缺氧组以终浓度150μmol/L的二氯化钴进行缺氧处理;48 h后分别采用qRT-PCR法和Western blotting法检测SMG-1 mRNA、蛋白。取缺氧处理的SKOV3细胞分为A、B、C、D组,A组为空白对照,B组转染阴性对照,C组仅加入转染试剂,D组转染SMG-1 siRNA。四组分别于转染48 h后测算细胞增殖抑制率、凋亡率,采用qRT-PCR法检测STAT3 mRNA,采用Western blotting法检测STAT3、p-STAT3蛋白。结果与常氧组相比,缺氧组SMG-1 mRNA及蛋白相对表达量升高(P均0.05)。以A组为参照(0),D组细胞增殖抑制率升高,与B、C组相比,P均0.05。与其余3组相比,D组细胞凋亡率升高,STAT3 mRNA及蛋白、p-STAT3蛋白相对表达量降低(P均0.05)。结论缺氧状态下卵巢癌SKOV3细胞中SMG-1表达上调;沉默SMG-1表达后SKOV3细胞增殖受到抑制、凋亡增多;SMG-1影响卵巢癌细胞增殖、凋亡的机制可能与改变STAT3的表达有关。
[Abstract]:Objective to investigate the changes of genitalia formation suppressor gene 1 (SMG-1) expression in ovarian cancer SKOV3 cells under hypoxia, and the effect of silencing SMG-1 on proliferation and apoptosis of SKOV3 cells, and to explore its mechanism. Methods Ovarian cancer SKOV3 cells were cultured and divided into normoxic group and anoxic group. The anoxia group was treated with cobalt dichloride at the final concentration of 150 渭 mol/L, and the SMG-1 mRNA, protein was detected by qRT-PCR and Western blotting assay 48 h later. SKOV3 cells treated with hypoxia were divided into two groups: group A was blank control, group B was negative control, group C was only adding transfection reagent, group D was transfection of SMG-1 siRNA.. The cell proliferation inhibition rate and apoptosis rate were measured 48 hours after transfection. STAT3 mRNA, was detected by qRT-PCR method and STAT3,p-STAT3 protein was detected by Western blotting assay. Results compared with normoxic group, the relative expression of SMG-1 mRNA and protein in hypoxia group was higher than that in normoxic group (P 0.05). The inhibitory rate of cell proliferation in group A (0), D) was higher than that in group C (P 0.05). Compared with the other three groups, the apoptosis rate of D group increased, and the relative expression of STAT3 mRNA, protein and p-STAT3 protein decreased (P0. 05). Conclusion the expression of SMG-1 was up-regulated in ovarian cancer SKOV3 cells under hypoxia, the proliferation of SKOV3 cells was inhibited and apoptosis increased after SMG-1 was silenced, and SMG-1 affected the proliferation of ovarian cancer cells. The mechanism of apoptosis may be related to the change of STAT3 expression.
【作者单位】：郑州大学第三附属医院;商丘医学高等专科学校;
【分类号】：R737.31

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