新型管式冷冻载体在人类胚胎库管理方面的应用

发布时间：2018-11-28 11:05

【摘要】：目的:针对现有玻璃化冷冻载体在胚胎库管理方面的不足,探讨新型管式冷冻载体的囊胚冷冻效果及其在人类胚胎库管理方面的应用。方法:选取D5、D6单囊胚玻璃化复苏移植的患者,其中杆式冷冻载体1257例,新型管式冷冻载体564例,比较复苏后的胚胎存活率及临床妊娠率。结果:杆式冷冻载体与管式冷冻载体存活胚胎率分别为99.05%(1245/1257)、100%(564/564),差异有统计学意义(χ~2=5.4199,P=0.0199),临床妊娠率分别为58.80%(732/1245)、60.28%(340/564),差异无统计学意义(χ~2=0.3562,P=0.5506)。管式冷冻载体相比杆式冷冻载体更节约空间,胚胎定位精确,取放速度更快。结论:与杆式冷冻载体比较,新型管式冷冻载体的临床妊娠率无显著差异,但在空间利用、位置准确性及拿取时间方面存在优势,更适用于实际的临床操作及胚胎库的管理。
[Abstract]:Aim: to investigate the effect of cryopreservation of blastocyst and its application in human embryo bank management in view of the deficiency of the existing vitrified cryopreservation carrier in embryo bank management. Methods: single blastocyst vitrification and resuscitation were performed in 1257 patients with D5 / D6 single blastocyst, including 1257 cases with rod cryopreservation carrier and 564 cases with new tube cryopreservation carrier. The embryo survival rate and clinical pregnancy rate after resuscitation were compared. Results: the embryo survival rates of rod and tube cryopreservation carriers were 99.05% (1245 / 1257) and 100% (564 / 564), respectively. The difference was statistically significant (蠂 ~ 2 ~ 2 ~ (5.4199) P ~ (0.0199). The clinical pregnancy rates were 58.80% (732 / 1245) and 60.28% (340 / 564), respectively. Compared with the rod freezing carrier, the tube cryopreservation carrier saves more space, the embryo location is accurate, and the extraction speed is faster. Conclusion: there is no significant difference in the clinical pregnancy rate of the new tube cryopreservation carrier compared with the rod cryopreservation carrier, but there are some advantages in space utilization, location accuracy and fetching time, which are more suitable for practical clinical operation and embryo bank management.
【作者单位】：山东大学生殖医学研究中心医院国家辅助生殖与优生工程技术研究中心生殖内分泌教育部重点实验室山东省生殖医学重点实验室;
【分类号】：R714.8

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