茶氨酸硝香酰胺对人宫颈癌细胞生长的抑制作用

发布时间：2018-12-17 12:27

【摘要】：前期研究发现本试验合成的茶氨酸衍生物茶氨酸硝香酰胺(TNC)具有显著抑制人肺癌细胞体内外生长的作用,为进一步探索研究TNC的抗癌活性和作用的分子机制,以其母体化合物茶氨酸为对照,评估现TNC对人宫颈癌细胞生长的抑制作用。采用MTT方法检测不同浓度的TNC对人宫颈癌细胞生长的影响,应用蛋白质印迹法检测解析这些癌细胞中与凋亡和生长密切相关蛋白的表达和药物可能作用的分子靶点。此外,通过建立动物肿瘤模型,评价TNC对荷瘤裸鼠体内人宫颈癌He La肿瘤生长的抑制效果。实验结果显示,TNC抑制人宫颈癌细胞体内外生长的活性超过其母体化合物茶氨酸多倍,对小鼠生长无明显毒性;TNC抑制人宫颈癌细胞生长的分子机制可能与抑制VEGFR2-NF-κB信号传导通路相关。本研究结果提示,TNC具有应用于临床治疗和(或)辅助治疗高转移人宫颈癌的潜力。
[Abstract]:In previous studies, it was found that theanine nitrovanamide (TNC), a derivative of theanine, could significantly inhibit the growth of human lung cancer cells in vitro and in vivo, and further explore the molecular mechanism of the anticancer activity and action of TNC. The inhibitory effect of TNC on the growth of human cervical cancer cells was evaluated with theanine as a control. The effects of different concentrations of TNC on the growth of human cervical cancer cells were detected by MTT. Western blot was used to detect and analyze the expression of proteins closely related to apoptosis and growth and the possible action of drugs in these cancer cells. In addition, animal tumor models were established to evaluate the inhibitory effect of TNC on the growth of human cervical cancer He La in nude mice. The results showed that the inhibitory activity of TNC on the growth of human cervical cancer cells in vivo and in vitro was more than that of its parent compound theanine, and had no obvious toxicity to mouse growth. The molecular mechanism of TNC inhibiting the growth of human cervical cancer cells may be related to the inhibition of VEGFR2-NF- 魏 B signal transduction pathway. The results suggest that TNC has the potential for clinical and / or adjuvant treatment of high metastatic cervical cancer.
【作者单位】：烟台大学药学院分子药理学实验室;
【基金】：国家科技部十二五“863”项目(2012AA020206) 山东省科技攻关项目(2009GG10002087) 山东省自然科学基金项目(ZR2015HM004)共同资助
【分类号】：R737.33

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