VEGF基因多态性与广西地区壮族妇女复发性自然流产的相关性研究

发布时间：2018-12-19 12:46

【摘要】：目的：复发性自然流产是妊娠初期最常见的并发症之一，其病因及发病机制及极繁杂，目前临床上尚无明确有效的预测和防治措施。本研究旨在探讨血管内皮生长因子(VEGF)的基因位点㧟2578C/A、㧟1154G/A、㧟634G/C、㧟460C/T和㧏936C/T的单核甘酸多态性(SNPs)与广西地区壮族妇女复发性自然流产(RSA)患者发病的关系，从基因水平探讨RSA发病的基因易感性，寻求与RSA相关的VEGF易感基因，进一步阐明RSA发病的分子生物学机制，为研究RSA的发病机理提供一种可行性的方法。方法：病例组选取门诊就诊的复发性流产患者共120例，对照组选取门诊就诊的健康经产妊娠妇女和住院待产的健康经产妊娠妇女共110例，两组入选观察对象均抽取静脉血，并采用传统手工苯酚-氯仿法提取全血DNA，采用PCR-限制性片段长度多态性方法(PCR-RFLP)检测两组观察对象VEGF基因5个多态位点的SNPs基因型的表达和频率分布情况。结果： VEGF㧟2578C/A、㧟1154G/A、㧟634G/C、㧟460C/T和㧏936C/T5个位点SNPs的各个基因型和等位基因频率表达分布在RSA组和对照组间差异均不显著，没有统计学意义(P＞0.05)。结论： VEGF㧟2578C/A、㧟1154G/A、㧟634G/C、㧟460C/T和㧏936C/T5个位点的基因多态性可能与广西地区壮族妇女复发性自然流产的发病易感性不相关联。研究结果与部分研究不同可能是多态性位点在不同地区，不同种族、民族，不同人群中的作用不一致所致。
[Abstract]:Objective: recurrent spontaneous abortion is one of the most common complications in early pregnancy. The aim of this study was to investigate the gene locus of vascular endothelial growth factor (VEGF), 2578 C / A, 1154 G / A, 634 G / C, ? 460C/T and? 936C/T mononuclear glycosylated (SNPs) were associated with the incidence of recurrent spontaneous abortion (RSA) in Zhuang women in Guangxi. The genetic susceptibility of RSA was investigated at the gene level, and the VEGF susceptibility genes associated with RSA were sought. Further elucidation of molecular biological mechanism of RSA provides a feasible method for studying the pathogenesis of RSA. Methods: 120 patients with recurrent abortion were selected in the case group, 110 healthy pregnant women in the outpatient department and 110 healthy pregnant women in the hospital were selected in the control group. Venous blood was drawn from the two groups. Whole blood DNA, was extracted by traditional manual phenol-chloroform method and PCR- restriction fragment length polymorphism (PCR-RFLP) was used to detect the expression and frequency distribution of SNPs genotypes in 5 polymorphic loci of VEGF gene in two groups. Results: the frequencies of SNPs genotypes and alleles in VEGF?2578C/A,?1154G/A,?634G/C,?460C/T and? 936C/T5 loci were not significantly different between RSA group and control group. There was no statistical significance (P > 0.05). Conclusion: the genetic polymorphisms of VEGF?2578C/A,?1154G/A,?634G/C,?460C/T and? 936C/T5 loci may not be associated with the susceptibility to recurrent spontaneous abortion in Zhuang women in Guangxi. The difference between the results and some studies may be due to the different roles of polymorphic loci in different regions, races, ethnic groups and different populations.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

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