miR-26a-5p通过靶向THAP2促进子宫内膜癌细胞凋亡

发布时间：2019-01-19 09:28

【摘要】：目的:研究miR-26a-5p靶向THAP2对子宫内膜癌细胞凋亡的影响。方法:实时荧光定量RT-PCR法检测子宫内膜癌细胞中miR-26a-5p表达水平。构建以慢病毒为载体的miR-26a-5p过表达和干扰质粒,分别转染子宫内膜癌细胞系Ishikawa与KLE,实时荧光定量RT-PCR法检测miR-26a-5p及下游分子THAP2转录水平。流式细胞技术分析miR-26a-5p表达对子宫内膜癌细胞凋亡的影响。结果:子宫内膜癌细胞Ishikawa与KLE均表达miR-26a-5p;miR-26a-5p过表达能增加THAP2的转录水平,促进子宫内膜癌细胞凋亡;miR-26a-5p下调能抑制THAP2的转录水平,抑制子宫内膜癌细胞凋亡。结论:miR-26a-5p可提高THAP2的转录水平,促进子宫内膜癌细胞凋亡,明确这种调控机制可能为子宫内膜癌预防诊断和治疗带来新的契机。
[Abstract]:Objective: to study the effect of miR-26a-5p targeted THAP2 on apoptosis of endometrial carcinoma cells. Methods: the expression of miR-26a-5p in endometrial carcinoma cells was detected by real-time fluorescence quantitative RT-PCR. MiR-26a-5p overexpression and interference plasmids were constructed and transfected into endometrial carcinoma cell line Ishikawa and KLE, real-time quantitative RT-PCR to detect the transcription level of miR-26a-5p and its downstream molecule THAP2. The effect of miR-26a-5p expression on apoptosis of endometrial carcinoma cells was analyzed by flow cytometry. Results: the overexpression of miR-26a-5p;miR-26a-5p in endometrial cancer cells both Ishikawa and KLE increased the transcription level of THAP2 and promoted the apoptosis of endometrial cancer cells. Down-regulation of miR-26a-5p can inhibit the transcription level of THAP2 and inhibit the apoptosis of endometrial cancer cells. Conclusion: miR-26a-5p can improve the transcription level of THAP2 and promote the apoptosis of endometrial cancer cells, which may bring a new opportunity for the prevention, diagnosis and treatment of endometrial carcinoma.
【作者单位】：上海交通大学附属第一人民医院;同济大学附属第一妇婴保健院;
【基金】：国家自然科学基金青年项目(No:81001154/H1621)
【分类号】：R737.33

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