高危型HPV DNA整合导致宫颈癌的作用机制和临床检测进展

发布时间：2019-01-20 16:05

【摘要】：高危型人乳头瘤病毒(human papillomavirus,HPV)感染是宫颈癌发生发展的必要因素之一。病毒DNA整合到宿主基因组中被认为是导致宫颈癌最重要的病因。高危型HPV DNA整合往往导致其E1和E2区大部分缺失或中断,E6和E7致癌基因过表达,宿主致癌基因激活和抑癌基因失活。目前研究表明高危型HPV整合可作为优质宫颈病变筛查的预测生物标志物,且其检测的有效方法大都基于荧光原位杂交、实时荧光定量PCR和杂交捕获技术结合Sanger测序法等。本文重点阐述了高危型HPV整合导致宫颈癌的主要机制,描述了宫颈病变筛查标志物以及预防性HPV疫苗研发和推广的研究进展,并综述了高危型HPV DNA整合状态的检测方法。
[Abstract]:High-risk human papillomavirus (human papillomavirus,HPV) infection is one of the necessary factors for the development of cervical cancer. The integration of viral DNA into the host genome is considered to be the most important cause of cervical cancer. High risk HPV DNA integration often leads to deletion or interruption of E1 and E2 regions, overexpression of E6 and E7 oncogenes, activation of host oncogenes and inactivation of tumor suppressor genes. Current studies have shown that high risk HPV integration can be used as a predictive biomarker for high quality cervical disease screening, and most of its effective methods are based on fluorescence in situ hybridization, real-time fluorescence quantitative PCR and hybridization capture technique combined with Sanger sequencing, and so on. This article focuses on the main mechanism of high risk HPV integration leading to cervical cancer, describes the research progress of screening markers for cervical lesions and the development and promotion of prophylactic HPV vaccine, and summarizes the detection methods of high risk HPV DNA integration status.
【作者单位】：广东医科大学;博奥生物集团有限公司转化医学研究院;深圳市第三人民医院检验科;
【基金】：深圳市科技研发资金项目深圳市生物芯片研究重点实验室项目资助(编号:ZDSYS201504301534057) 深圳市三名工程专项基金项目资助~~
【分类号】：R737.33

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