人乳头状瘤病毒感染与宫颈癌组织中核转录因子κB表达的关系研究

发布时间：2019-01-21 12:52

【摘要】：目的探讨人乳头状瘤病毒(HPV)感染与宫颈癌组织中核转录因子κB(NF-κB)表达的关系,为进一步探索宫颈癌发生的原因以及研究相应的药物治疗提供试验依据。方法将医院2012年6月-2014年1月妇产门诊或住院收治的56例宫颈癌患者,同期收集对照组36例子宫肌瘤患者,采用免疫细胞化学染色法检测患者组织中NF-κB/p65、磷酸化核因子-κB(P-NF-κB/p65)蛋白的表达。结果 NF-κB/p65、P-NF-κB/p65的表达随宫颈癌病变的级别升高而升高,与宫颈癌病变级别呈正相关,差异有统计学意义(P0.05);在NF-κB/p65、P-NF-κB/p65表达阳性的宫颈癌组织中,HPV16型阳性组的NF-κB表达的染色分值明显高于HPV16型阴性组,差异有统计学意义(P0.05),提示HPV感染可以诱导NF-κB活化,NF-κB可能参与HPV16型的致癌过程,其表达随着宫颈病变的升高而升高,与宫颈病呈正相关;NF-κB/p65、P-NF-κB/p65在慢性宫颈炎组、CIN组和宫颈癌组的表达差异有统计学意义(χ2=24.39,P0.05);NF-κB/p65、P-NF-κB/p65在宫颈癌的共表达差异有统计学意义(χ2=12.58,P0.05)。结论人乳头状瘤病毒感染能促进NF-κB/p65、P-NF-κB/p65的表达和激活,NF-κB/p65、P-NF-κB/p65可能参与宫颈癌的发生和发展。
[Abstract]:Objective to investigate the relationship between (HPV) infection of human papillomavirus (HPV) and the expression of NF- 魏 B in cervical carcinoma, and to provide experimental evidence for further exploration of the pathogenesis of cervical cancer and the study of corresponding drug therapy. Methods from June 2012 to January 2014, 56 patients with cervical cancer were collected from outpatient or inpatient department of obstetrics and gynecology, and 36 patients with uterine leiomyoma were collected from the control group. NF- 魏 B / p65 was detected by immunocytochemical staining. Expression of phosphorylated nuclear factor- 魏 B (P-NF- 魏 B/p65) protein. Results the expression of NF- 魏 B / p65 P-NF- 魏 B/p65 increased with the grade of cervical cancer, and was positively correlated with the grade of cervical cancer (P0.05). In cervical cancer tissues with positive expression of P-NF- 魏 B/p65, the staining score of NF- 魏 B in HPV16 positive group was significantly higher than that in HPV16 negative group (P0.05). The results suggest that HPV infection can induce the activation of NF- 魏 B, and NF- 魏 B may be involved in the carcinogenesis of HPV16 type. The expression of NF- 魏 B may increase with the increase of cervical lesions, which is positively correlated with cervical disease. The expression of NF- 魏 B / p65 P NF- 魏 B/p65 in chronic cervicitis group, CIN group and cervical cancer group was significantly different (蠂 2 + 24.39 P 0.05). The coexpression of NF- 魏 B / p65 P NF- 魏 B/p65 in cervical carcinoma was significantly different (蠂 2: 12.58 P 0.05). Conclusion Human papillomavirus infection can promote the expression and activation of NF- 魏 B / p65 P-NF- 魏 B/p65. NF- 魏 B / p65 P-NF- 魏 B/p65 may be involved in the occurrence and development of cervical cancer.
【作者单位】：聊城市复退军人医院妇产科;
【基金】：山东省卫生厅基金资助项目(JB2008卫-2-19-1)
【分类号】：R737.33

【参考文献】