PPARγ在绒毛组织中的表达与早期自然流产的关系

发布时间：2019-01-21 20:25

【摘要】：自然流产是妊娠最常见疾病,目前据统计,31%的胚胎着床后发生自然流产,其中80%为早期流产[1],其病因复杂多样,确切的发病机制目前仍不清楚。近年来人们对核内受体转录因子超家族成员过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor-γ,PPARγ)的研究发现其在妊娠期胎盘形成、发育过程中发挥着不可或缺的作用,在多种妊娠相关疾病中表达异常,在今后的研究中有望将其作为治疗和阻止不良妊娠结局一个有潜力的治疗靶点。目的:研究PPARγ在正常早孕及早期自然流产患者绒毛中的差异表达,由此分析两者可能存在的联系,进一步探讨PPARγ与早期自然流产的关系。方法:应用免疫组织化学(Power Vision,PV)两步法检测PPARγ蛋白在40例正常早孕(对照组)和40例早期自然流产患者(实验组)绒毛组织中是否有表达;应用Western Blot、RT-PCR技术检测PPARγ蛋白及基因在两组绒毛组织中的表达水平,并分析是否有显著性差异。两组实验对象均无妊娠合并症及并发症,既往否认不良孕产史,术前1个月未服用激素类药物,临床特征无显著差异,资料具有比较性。结果:1.免疫组化显示PPARγ蛋白主要在两组患者绒毛组织中细胞滋养层细胞及绒毛外滋养层细胞中均有表达,且实验组PPARγ表达水平升高,差异有统计学意义(P0.05);2.Western Blot分析显示PPARγ蛋白在实验组患者绒毛组织中的表达较对照组高,统计学差异显著(P0.01)。3.RT-PCR分析显示PPARγ基因在实验组患者绒毛组织中的表达较对照组高,统计学差异显著(P0.01)。结论:1.PPARγ蛋白主要在早孕及自然流产患者绒毛组织中细胞滋养层细胞及绒毛外滋养层细胞中表达;2.PPARγ蛋白及mRNA在早期自然流产患者绒毛组织中的表达较正常早孕明显升高,可能与自然流产的发生有着密切关联。
[Abstract]:Spontaneous abortion is the most common disease of pregnancy. According to statistics, 31% of the embryos have spontaneous abortion after implantation, 80% of which are early abortion [1], its etiology is complex and diverse, and the exact pathogenesis is still unclear. In recent years, peroxisome proliferator-activated receptor- 纬 (PPAR 纬), a member of the nuclear receptor transcription factor superfamily, has been found to play an indispensable role in placenta formation and development during pregnancy. Abnormal expression in various pregnancy-related diseases is expected to be a potential therapeutic target for the treatment and prevention of adverse pregnancy outcomes in future research. Objective: to study the differential expression of PPAR 纬 in villi of normal early pregnancy and early spontaneous abortion, and to analyze the possible relationship between PPAR 纬 and early spontaneous abortion. Methods: the expression of PPAR 纬 protein in the villi of 40 normal early pregnant women (control group) and 40 early spontaneous abortion patients (experimental group) was detected by two step immunohistochemical method (Power Vision,PV). Western Blot,RT-PCR technique was used to detect the expression of PPAR 纬 protein and gene in chorionic villi of the two groups, and to analyze whether there were significant differences between the two groups. There was no complication of pregnancy and complications in the two groups. The history of adverse pregnancy and childbirth was denied. The patients did not take steroid drugs 1 month before operation. There was no significant difference in clinical characteristics between the two groups. Results: 1. Immunohistochemical staining showed that PPAR 纬 protein was mainly expressed in trophoblast cells and extravillus trophoblastic cells in the two groups, and the expression level of PPAR 纬 in the experimental group was higher than that in the control group (P0.05). 2.Western Blot analysis showed that the expression of PPAR 纬 protein in the chorionic villi of the experimental group was higher than that in the control group (P0.01). 3.RT-PCR analysis showed that the expression of PPAR 纬 gene in the chorionic villi of the experimental group was higher than that in the control group. The difference was significant (P0.01). Conclusion: 1.PPAR 纬 protein is mainly expressed in cytotrophoblast cells and extravillous trophoblast cells in villi of early pregnancy and spontaneous abortion. The expression of 2.PPAR 纬 protein and mRNA in villi of early spontaneous abortion patients was significantly higher than that of normal early pregnancy, which may be closely related to the occurrence of spontaneous abortion.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

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