TGA与LPS竞争抑制妊娠期糖尿病孕妇外周血单核细胞TLR4经典信号通路

发布时间：2019-01-24 10:50

【摘要】：目的研究半乳糖醛酸(TGA)与内毒素(LPS)同时刺激妊娠期糖尿病孕妇外周血单核细胞及其对LPS-Toll样受体4(TLR-4)-核转录因子-κB(NF-κB)信号通路的抑制作用。方法选取妊娠期糖尿病孕妇30例,抽取外周静脉血15 ml,分离出单核细胞,分别加入LPS、TGA、TGA(0~1.0mg/ml)联合LPS刺激并培养,Western blot法检测TLR4及NF-κB p65蛋白表达量,ELISA法检测培养液中肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-10(IL-10)水平。结果单独LPS或TGA刺激,其下游TLR4、NF-κB p65蛋白表达量及TNF-α、IL-1、IL-10水平均较未处理组高,差异有统计学意义(P0.05);同时加入TGA及LPS刺激,其TLR4、NF-κB p65蛋白表达量及TNF-α、IL-1、IL-10水平均较单独LPS或TGA刺激低,但较未处理组高,差异均有统计学意义(P0.05);各组TLR4与NF-κB p65蛋白表达量之间呈正相关性(P0.01)。结论 TGA与LPS可竞争抑制LPS-TLR4-NF-κB信号通路,对妊娠期糖尿病的预防及治疗起到指导作用。
[Abstract]:Objective to study the stimulation of monocyte in peripheral blood of pregnant women with gestational diabetes mellitus (GDM) by (TGA) and endotoxin (LPS) and its inhibitory effect on the signal pathway of LPS-Toll like receptor 4 (TLR-4)-nuclear transcription factor 魏 B (NF- 魏 B). Methods monocytes were isolated from 30 pregnant women with gestational diabetes mellitus and peripheral venous blood was isolated for 15 ml,. The expression of TLR4 and NF- 魏 B p65 protein were detected by, Western blot and LPS,TGA,TGA (0~1.0mg/ml) combined with LPS stimulation. The levels of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 (IL-1) and interleukin-10 (IL-10) were measured by ELISA. Results the expression of TLR4,NF- 魏 B p65 protein and the levels of TNF- 伪 and IL-1,IL-10 in the downstream of LPS or TGA alone were significantly higher than those in the untreated group (P0.05). The expression of TLR4,NF- 魏 B p65 protein and the levels of TNF- 伪 and IL-1,IL-10 were lower than those of LPS or TGA alone, but higher than those of untreated group (P0.05). There was a positive correlation between TLR4 and p65 expression of NF- 魏 B (P0.01). Conclusion TGA and LPS can inhibit LPS-TLR4-NF- 魏 B signaling pathway and play a guiding role in the prevention and treatment of gestational diabetes mellitus.
【作者单位】：安徽医科大学第一附属医院妇产科产前诊断中心;
【基金】：安徽省自然科学基金(编号:1208085MH172) 安徽高校省级自然科学研究项目(编号:KJ2011Z215)
【分类号】：R714.256

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