子痫前期患者血清中基质金属蛋白酶2和9及其抑制因子的水平研究

发布时间：2019-01-26 21:04

【摘要】：目的 :研究子痫前期患者血清中的基质金属蛋白酶(matrix metalloproteinases,MMP)-2和MMP-9及其抑制因子(tissue inhibitors of matrix metalloproteinases,TIMP)2与TIMP1的表达水平。方法 :选取88例妊娠妇女,其中25例妊娠高血压、18例子痫前期轻度患者、15例子痫前期重度患者及30例正常妊娠者,采用ELISA法检测血清中的MMP-2、MMP-9、TIMP1及TIMP2的表达水平,并计算MMP-2净含量(MMP-2/TIMP2)和MMP-9净含量(MMP-9/TIMP1)。结果:子痫前期患者血清中MMP-2含量显著增高。子痫前期重度组含量达(2.58±0.33)ng/ml,该组MMP-2含量及净含量(MMP-2/TIMP2)较其他各组均显著增加(P0.05)。相反,子痫前期患者血清中MMP-9含量明显降低,而正常妊娠组MMP-9含量为(0.79±0.20)ng/ml,其MMP-9含量及净含量(MMP-9/TIMP1)较其他各组均增高(P0.05)。结论 :子痫前期患者血清中MMP-2表达增加,MMP-2可能是妊娠高血压疾病的主要相关因子。
[Abstract]:Aim: to study the expression of matrix metalloproteinase (matrix metalloproteinases,MMP)-2, MMP-9 and its inhibitor (tissue inhibitors of matrix metalloproteinases,TIMP) 2 and TIMP1 in serum of preeclampsia patients. Methods: MMP-2,MMP-9, in serum of 88 pregnant women, including 25 cases of pregnancy hypertension, 18 cases of mild preeclampsia, 15 cases of severe preeclampsia and 30 cases of normal pregnancy, were detected by ELISA method. The expression levels of TIMP1 and TIMP2, and the net content of MMP-2 (MMP-2/TIMP2) and MMP-9 (MMP-9/TIMP1) were calculated. Results: the content of MMP-2 in serum of preeclampsia patients was significantly increased. The content of MMP-2 and net content (MMP-2/TIMP2) of severe preeclampsia group was (2.58 卤0.33) ng/ml, which was significantly higher than that of other groups (P0.05). On the contrary, the serum MMP-9 content of preeclampsia patients was significantly lower, while the MMP-9 content of normal pregnancy group was (0.79 卤0.20) ng/ml,. The MMP-9 content and net content (MMP-9/TIMP1) of normal pregnancy group were higher than those of other groups (P0.05). Conclusion: the expression of MMP-2 in serum of preeclampsia patients is increased, and MMP-2 may be the main related factor of pregnancy-induced hypertension.
【作者单位】：苏州市市立医院东区妇产科;
【基金】：苏州市科技计划项目(SYS201048)
【分类号】：R714.244

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