LOXL1基因多态性及β-catenin表达与盆腔脏器脱垂的关系
发布时间：2019-01-27 13:53
【摘要】：背景： 盆腔脏器脱垂（Pelvic Organ Prolapse, POP）是中老年妇女中的常见妇科疾病，对患者的健康和生活质量有着严重影响，随着社会老龄化进程的加快，POP受到的关注与日俱增。 POP的病因复杂，涉及多个基因的改变和多种mRNA及其蛋白质的表达。本实验研究了LOXL1基因单核苷酸多态性（single nucleotide polymorphism,SNPs）以及β-catenin mRNA的表达与POP的关系，初步探讨了它们在POP发病机制中的作用。 目的： 研究LOXL1基因SNPs以及β-catenin mRNA的表达与POP发生、发展之间的关系，从分子水平进一步解释盆腔脏器脱垂的病因学机制。 方法： 选择盆腔脏器脱垂患者29例，同时选择无POP的良性妇科疾病患者29例作为对照组。从全血中提取基因组DNA，对LOXL1基因片段进行扩增，对扩增产物进行直接测序，分析rs1048661位点及rs3825942位点的基因多态性。 选择因盆腔脏器脱垂行全子宫切除术的患者17例，同时选择无POP的、因良性妇科疾病行全子宫切除术的患者17例作为对照组。取主韧带组织2块，1块用于提取RNA，使用RT-PCR技术获得β-catenin的cDNA,将产物进行电泳并计算相对吸光度。另1块制成组织切片，使用丽春红-苦味酸-维多利亚蓝染色法进行染色，观察其中胶原纤维及弹性纤维的含量。 结果： 1、rs1048661位点SNPs，盆腔脏器脱垂患者的等位基因构成比、基因型构成比与对照组无统计学差异（P值均0.05）。在rs3825942位点，盆腔脏器脱垂组等位基因G的构成比显著高于对照组，，P=0.029，两组间差异具有统计学意义。无论是盆腔脏器脱垂患者组还是对照组，均未在rs3825942位点发现AA基因型。盆腔脏器脱垂患者GG基因型频率显著高于对照组，P=0.021。 2、盆腔脏器脱垂患者主韧带中β-catenin mRNA含量显著低于对照组，P=0.02。 3、盆腔脏器脱垂患者子宫主韧带组织中胶原纤维及弹性纤维相对吸光度值明显均低于对照组（P=0.010，P=0.006），差异有统计学意义。β-catenin mRNA在子宫主韧带中的表达量与子宫主韧带中的胶原纤维含量之间存在直线回归关系，P 0.01；而β-catenin mRNA的表达量与弹性纤维的含量之间，不存在直线回归关系，P0.05。 结论： 1、LOXL1的单核苷酸多态性与中国汉族女性盆腔脏器脱垂的发病有关。 2、 rs3825942单核苷酸多态性位点，等位基因G是盆腔脏器脱垂的危险等位基因，GG基因型是盆腔脏器脱垂的危险基因型。 3、盆腔脏器脱垂组患者子宫主韧带组织中β-catenin mRNA表达量下降。 4、盆腔脏器脱垂组患者主韧带中的弹性纤维和胶原纤维含量明显降低。 5、子宫主韧带中β-catenin mRNA表达量降低与胶原纤维含量降低呈直线相关关系。
[Abstract]:Background: pelvic viscera prolapse (Pelvic Organ Prolapse, POP) is a common gynecological disease in middle-aged and elderly women, which has a serious impact on patients' health and quality of life. With the acceleration of the aging process of society, POP is receiving increasing attention. The etiology of POP is complex, involving multiple gene changes and the expression of multiple mRNA and its proteins. In this study, we studied the relationship between LOXL1 gene single nucleotide polymorphisms (single nucleotide polymorphism,SNPs), 尾-catenin mRNA expression and POP, and discussed their roles in the pathogenesis of POP. Objective: to study the relationship between the expression of SNPs and 尾-catenin mRNA of LOXL1 gene and the occurrence and development of POP, and to further explain the etiological mechanism of pelvic organ prolapse at molecular level. Methods: 29 patients with pelvic organ prolapse and 29 patients with benign gynecological diseases without POP were selected as control group. Genomic DNA, was extracted from whole blood to amplify the LOXL1 gene fragment. The amplified product was sequenced directly and the polymorphism of rs1048661 locus and rs3825942 locus was analyzed. Seventeen patients with pelvic organ prolapse underwent total hysterectomy, 17 patients without POP and 17 patients with benign gynecologic diseases were selected as control group. The main ligaments were extracted from two pieces of tissue and one piece was used to extract RNA,. The 尾-catenin cDNA, was obtained by RT-PCR technique. The product was electrophoretic and the relative absorbance was calculated. The other one was made into tissue section and stained with Victorian blue staining method. The contents of collagen and elastic fibers were observed. Results: 1 the allelic and genotypic ratios of patients with SNPs, pelvic organ prolapse at rs1048661 were not significantly different from those of the control group (P < 0. 05). At the rs3825942 locus, the G ratio of allele G in pelvic organ prolapse group was significantly higher than that in control group, and the difference between the two groups was statistically significant. Neither the pelvic organ prolapse group nor the control group found AA genotypes at rs3825942 locus. The frequency of GG genotype in patients with pelvic organ prolapse was significantly higher than that in control group (P < 0.021). 2, the content of 尾-catenin mRNA in the main ligament of pelvic viscera prolapse patients was significantly lower than that in the control group (P < 0. 02). 3. The relative absorbance of collagen fiber and elastic fiber in the main ligament of uterus in patients with prolapse of pelvic viscera was significantly lower than that in the control group (P0. 010, P0. 006). There was a linear regression relationship between the expression of 尾-catenin mRNA in the main uterine ligament and the content of collagen fiber in the main uterine ligament (P 0.01). However, there was no linear regression relationship between the expression of 尾-catenin mRNA and the content of elastic fiber, P0.05. Conclusion: 1 the single nucleotide polymorphism of LOXL1 is associated with pelvic organ prolapse in Chinese Han women. 2, rs3825942 single nucleotide polymorphism locus, allele G is the dangerous allele of pelvic organ prolapse, GG genotype is the dangerous genotype of pelvic organ prolapse. 3. The expression of 尾-catenin mRNA in the main ligament of uterus decreased in pelvic organ prolapse group. 4. The content of elastic fiber and collagen fiber in the main ligament of pelvic viscera prolapse group was significantly decreased. 5. There was a linear correlation between the decrease of 尾-catenin mRNA expression and the decrease of collagen fiber content in the main ligament of uterus.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.2
本文编号：2416323
[Abstract]:Background: pelvic viscera prolapse (Pelvic Organ Prolapse, POP) is a common gynecological disease in middle-aged and elderly women, which has a serious impact on patients' health and quality of life. With the acceleration of the aging process of society, POP is receiving increasing attention. The etiology of POP is complex, involving multiple gene changes and the expression of multiple mRNA and its proteins. In this study, we studied the relationship between LOXL1 gene single nucleotide polymorphisms (single nucleotide polymorphism,SNPs), 尾-catenin mRNA expression and POP, and discussed their roles in the pathogenesis of POP. Objective: to study the relationship between the expression of SNPs and 尾-catenin mRNA of LOXL1 gene and the occurrence and development of POP, and to further explain the etiological mechanism of pelvic organ prolapse at molecular level. Methods: 29 patients with pelvic organ prolapse and 29 patients with benign gynecological diseases without POP were selected as control group. Genomic DNA, was extracted from whole blood to amplify the LOXL1 gene fragment. The amplified product was sequenced directly and the polymorphism of rs1048661 locus and rs3825942 locus was analyzed. Seventeen patients with pelvic organ prolapse underwent total hysterectomy, 17 patients without POP and 17 patients with benign gynecologic diseases were selected as control group. The main ligaments were extracted from two pieces of tissue and one piece was used to extract RNA,. The 尾-catenin cDNA, was obtained by RT-PCR technique. The product was electrophoretic and the relative absorbance was calculated. The other one was made into tissue section and stained with Victorian blue staining method. The contents of collagen and elastic fibers were observed. Results: 1 the allelic and genotypic ratios of patients with SNPs, pelvic organ prolapse at rs1048661 were not significantly different from those of the control group (P < 0. 05). At the rs3825942 locus, the G ratio of allele G in pelvic organ prolapse group was significantly higher than that in control group, and the difference between the two groups was statistically significant. Neither the pelvic organ prolapse group nor the control group found AA genotypes at rs3825942 locus. The frequency of GG genotype in patients with pelvic organ prolapse was significantly higher than that in control group (P < 0.021). 2, the content of 尾-catenin mRNA in the main ligament of pelvic viscera prolapse patients was significantly lower than that in the control group (P < 0. 02). 3. The relative absorbance of collagen fiber and elastic fiber in the main ligament of uterus in patients with prolapse of pelvic viscera was significantly lower than that in the control group (P0. 010, P0. 006). There was a linear regression relationship between the expression of 尾-catenin mRNA in the main uterine ligament and the content of collagen fiber in the main uterine ligament (P 0.01). However, there was no linear regression relationship between the expression of 尾-catenin mRNA and the content of elastic fiber, P0.05. Conclusion: 1 the single nucleotide polymorphism of LOXL1 is associated with pelvic organ prolapse in Chinese Han women. 2, rs3825942 single nucleotide polymorphism locus, allele G is the dangerous allele of pelvic organ prolapse, GG genotype is the dangerous genotype of pelvic organ prolapse. 3. The expression of 尾-catenin mRNA in the main ligament of uterus decreased in pelvic organ prolapse group. 4. The content of elastic fiber and collagen fiber in the main ligament of pelvic viscera prolapse group was significantly decreased. 5. There was a linear correlation between the decrease of 尾-catenin mRNA expression and the decrease of collagen fiber content in the main ligament of uterus.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.2
【参考文献】
中国期刊全文数据库 前4条
1 张琳琳;于浩;张师前;;绝经前盆腔器官膨出患者子宫主韧带中Elastin、LOX基因表达的研究[J];山东大学学报(医学版);2008年02期
2 龙素萍;张振武;李义星;;MMP-3和CollagenⅢ在盆底器官脱垂患者阴道壁组织中表达的研究[J];中国医药指南;2008年19期
3 任琛琛;;POP患者盆底组织中MMP-3、TIMP-3的研究[J];中国妇幼保健;2008年20期
4 朱兰,郎景和,冯瑞娥,陈杰;绝经后压力性尿失禁患者盆底支持结构雌激素受体的研究[J];中华妇产科杂志;2004年10期
本文编号：2416323
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