子痫前期相关miR-18a对人正常滋养细胞浸润功能影响的研究

发布时间：2019-01-30 19:03

【摘要】：目的通过研究子痫前期相关微小RNA-18a(micro RNA-18a,mi R-18a)对人正常滋养细胞(HTR8)浸润功能的影响,探讨mi R-18a与子痫前期疾病的相关性及其在发病过程中的作用机制。方法将mi R-18a前体分子分组转染HTR8细胞,并分为三组:pre-mi RNA阴性对照组(NC)、mi R-18a过表达组和mi R-18a抑制组;反转录PCR检测转染后各组中mi R-18a m RNA的表达水平;侵袭小室实验(Transwell)检测转染后各组细胞浸润能力的变化情况。结果与NC组(0.407±0.019)比较,mi R-18a过表达组抑制效果显著(0.884±0.061;0.161±0.014),差异具有统计学意义(P0.05);与NC组(66.67±3.05)比较,抑制mi R-18a的表达可明显降低细胞的浸润能力(29.631±3.112,P0.05)。结论 mi R-18a能够在一定程度上影响人正常滋养细胞的浸润功能,可能与子痫前期的发病相关。
[Abstract]:Objective to study the effect of preeclampsia associated microRNA-18a (micro RNA-18a,mi R-18a) on the infiltration of human normal trophoblast (HTR8). To investigate the relationship between mi R-18a and preeclampsia and its role in the pathogenesis of preeclampsia. Methods mi R-18a precursor molecules were transfected into HTR8 cells and divided into three groups: pre-mi RNA negative control group, (NC), mi R-18a overexpression group and mi R-18a inhibition group; Reverse transcription PCR was used to detect the expression level of mi R-18a m RNA in each group, and invasive chamber experiment (Transwell) was used to detect the change of cell infiltration ability after transfection. Results compared with NC group (0.407 卤0.019), the inhibitory effect of mi R-18a overexpression group was significant (0.884 卤0.061 卤0.161 卤0.014), the difference was statistically significant (P0.05). Compared with NC group (66.67 卤3.05), inhibiting the expression of mi R-18a significantly decreased the infiltrating ability of cells (29.631 卤3.112 P 0.05). Conclusion mi R-18a can affect the infiltration function of human normal trophoblast to some extent and may be related to the pathogenesis of preeclampsia.
【作者单位】：西安医学院第一附属医院妇科;
【分类号】：R714.244

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