宫颈癌中结缔组织生长因子的表达及其相关因素分析

发布时间：2019-02-13 09:10

【摘要】：目的探讨结缔组织生长因子(CTGF)在子宫颈癌组织中的表达水平及其与临床病理因素间的关系。方法用实时荧光定量聚合链式反应和免疫印迹法检测61例子宫颈癌癌组织(试验组)和对应癌旁正常宫颈组织(对照组)中CTGF mRNA及蛋白的表达水平,并进行多因素Logistic回归分析。结果对照组和试验组的CTGF mRNA表达量分别为(1.00±0.01)和(0.30±0.03),CTGF蛋白的表达量分别为(0.98±0.02)和(0.22±0.05),差异均有统计学意义(均P0.01)。多因素Logistic回归分析结果显示:CTGF mRNA表达与宫颈癌的FIGO分期、淋巴结转移和分化程度呈负相关,CTGF蛋白表达与FIGO分期呈负相关。结论 CTGF在宫颈癌中低表达,可能作为抑癌因子在宫颈癌的发生发展中发挥作用。
[Abstract]:Objective to investigate the expression of connective tissue growth factor (CTGF) in cervical carcinoma and its relationship with clinicopathological factors. Methods the expression levels of CTGF mRNA and protein in 61 cases of cervical cancer tissues (trial group) and corresponding normal cervical tissues (control group) were detected by real-time fluorescence quantitative polymerization chain reaction and Western blotting. Multivariate Logistic regression analysis was performed. Results the expression of CTGF mRNA in control group and experimental group were (1.00 卤0.01) and (0.30 卤0.03), CTGF, respectively (0.98 卤0.02) and (0.22 卤0.05), the difference was statistically significant (P0.01). Multivariate Logistic regression analysis showed that the expression of: CTGF mRNA was negatively correlated with the FIGO stage, lymph node metastasis and differentiation of cervical cancer, and the expression of CTGF protein was negatively correlated with FIGO stage. Conclusion the low expression of CTGF may play an important role as a tumor suppressor in the occurrence and development of cervical cancer.
【作者单位】：承德医学院附属医院肿瘤内科;承德医学院基础医学院电镜室;承德医学院附属医院妇科;
【基金】：河北省卫计委医学科学研究重点课题计划基金资助项目(20150015) 承德市科技局科学研究与发展计划基金资助项目(20122148,20142031)
【分类号】：R737.33

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