MiR-195及IKBKB蛋白在子痫前期患者胎盘组织中的表达及意义

发布时间：2019-02-14 20:55

【摘要】：目的:探讨mi R-195及IKBKB蛋白在子痫前期患者胎盘组织中的表达及意义。方法:分别选择2015年08月份到2016年06月份期间在山西医科大第二附属医院的产科的住院的并生产的PE患者各30例,分别采集相应的临床资料以及收集胎盘组织;应用RT-qPCR法检测胎盘中的miR-195的相对表达水平;应用免疫组织化学法以及Western-blot法检测胎盘组织IKBKB蛋白的表达;使用SPSS 17.0软件进行统计学处理。结果:(1)研究组与正常对照组在年龄(t=1.86)和体重指数(t=0.92)之间无统计学意义(P0.05)。两组分娩时的孕周[(32.8±0.77)vs(39.02±0.69),t=6.24,P(27)0.01]、收缩压[(180.9±9.2)vs(118.6±3.27),t=7.38,P(27)0.01]、舒张压[(118.7±6.02)vs(69.2±2.18),t=8.82,P(27)0.01]、新生儿出生体重[(1737±201.3)vs(2870±193.2),t=3.84,P(27)0.01]之间差异有统计学意义(P0.01);(2)子痫前期患者miR-195在子痫前期研究组胎盘中的相对表达量为正常孕妇组的(0.36±0.20)倍;(3)生物信息学分析显示IKBKB可能为miR-195的靶基因;(4)子痫前期研究组胎盘滋养细胞中IKBKB蛋白的表达(OD:0.08±0.01)显著低于正常妊娠对照组(OD:0.13±0.01),差异有统计学意义(t=11.63,P0.001);(5)Western-blot检测IKBKB蛋白显示子痫前期研究组胎盘滋养细胞中IKBKB蛋白的相对表达量(0.08±0.01)显著低于正常妊娠对照组(0.13±0.01),差异有统计学意义(t=13.94,P0.001);(6)相关性分析表明本实验中IKBKB与miR-195无相关性(r=0.1227,P=0.5503)。结论:子痫前期患者胎盘组织中miR-195与IKBKB蛋白表达水平下调,提示miR-195及IKBKB蛋白可能通过调节滋养细胞凋亡参与子痫前期的发生和发展。
[Abstract]:Objective: to investigate the expression and significance of mi R 195 and IKBKB protein in placenta of preeclampsia. Methods: from August 2015 to June 2016, 30 cases of PE patients who were hospitalized and delivered in the second affiliated Hospital of Shanxi Medical University were selected, and the corresponding clinical data and placenta tissue were collected. The relative expression of miR-195 in placenta was detected by RT-qPCR method, the expression of IKBKB protein in placenta was detected by immunohistochemical method and Western-blot method, and the expression of IKBKB protein was analyzed by SPSS 17.0 software. Results: (1) there was no significant difference in age (t = 1.86) and body mass index (t = 0.92) between the study group and the normal control group (P0.05). The gestational weeks at delivery in both groups [(32.8 卤0.77) vs (, 39.02 卤0.69), 6.24) vs (P (27) 0.01], SBP [(180.9 卤9.2) vs (118.6 卤3.27), t 7.38) vs (P (27) 0.01]. Diastolic blood pressure (DBP) [(118.7 卤6.02) vs (, 69.2 卤2.18), t = 8.82) vs (, P (27) 0.01], neonatal birth weight [(1737 卤201.3) vs (, 2870 卤193.2), t = 3.84], P (27) 0.01] the difference was statistically significant (P0.01). (2) the relative expression of miR-195 in placenta of preeclampsia study group was 0.36 卤0.20 times higher than that of normal pregnant women, (3) bioinformatics analysis showed that IKBKB might be the target gene of miR-195. (4) the expression of IKBKB protein in placental trophoblast of preeclampsia study group (OD:0.08 卤0. 01) was significantly lower than that of normal pregnancy group (OD:0.13 卤0. 01). (5) the relative expression of IKBKB protein in placental trophoblastic cells of preeclampsia study group (0.08 卤0.01) was significantly lower than that of normal pregnancy group (0.13 卤0.01), and the difference was statistically significant (t _ (13) 94). (P0.001); (6) correlation analysis showed that there was no correlation between IKBKB and miR-195 in this experiment (r = 0.1227). Conclusion: the expression of miR-195 and IKBKB proteins in placenta of preeclampsia patients is down-regulated, suggesting that miR-195 and IKBKB proteins may be involved in the occurrence and development of preeclampsia by regulating the apoptosis of trophoblast.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244

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