孕期酒精暴露抑制低氧环境下新生大鼠耗氧量和基本节律性呼吸放电

发布时间：2019-02-24 10:01

【摘要】：目的研究孕期酒精暴露对低氧环境下新生大鼠在体耗氧量和离体延髓脑片基本节律性呼吸放电(RRDA)有无抑制作用。方法成年大鼠分为酒精暴露组和对照组,酒精暴露组从合笼前3天开始以8%酒精水溶液为唯一饮用水源,对照组正常饮食,其余饲养条件相同,使用两组孕鼠产下的新生大鼠:1采用体积描记法测量2~10 d新生大鼠在空气、15%氧浓度、10%氧浓度、5%氧浓度下单位时间内耗氧量,比较两组新生大鼠在不同氧浓度下耗氧量有无差异;2使用2 d新生大鼠制作离体延髓脑片标本,记录RRDA,依次使用经95%氧O2+5%二氧化碳CO2、80%O2+4.2%CO2+15.8%氮N2、65%O2+3.4%CO2+31.6%N2、50%O2+2.6%CO2+47.4%N2充分饱和的0℃改良kreb’s(MKS)从高氧到低氧灌流脑片,记录并分析各组脑片RRDA的改变。结果 1在体耗氧量实验显示在空气、15%氧浓度、10%氧浓度下两组新生大鼠单位时间内耗氧量差异无统计学意义,在5%氧浓度下,酒精暴露组耗氧量低于对照组。2酒精暴露组脑片放电弱于对照组,即RRDA的吸气时程(TI)、呼吸周期(RC)、放电积分幅度(IA)均弱于对照组。3随着灌流液氧浓度下降,两组脑片放电均逐渐减弱,TI缩短、RC延长、IA降低,酒精暴露组RRDA降低程度大于对照组。结论 1孕期酒精暴露抑制新生大鼠在低氧浓度下摄取氧的能力及离体延髓脑片RRDA。2两组新生大鼠整体耗氧量在5%氧浓度下差异有统计学意义,而离体脑片放电在80%氧浓度即下降的实验结果间接证实外周化学感受器对呼吸有着重要的调节作用。
[Abstract]:Objective to study the inhibitory effects of alcohol exposure during pregnancy on oxygen consumption in vivo and basic rhythmic respiratory discharge (RRDA) in isolated medulla oblongata slices of neonatal rats in hypoxic environment. Methods Adult rats were divided into two groups: alcohol exposure group and control group. The alcohol exposure group was treated with 8% alcohol solution as the only drinking water source from 3 days before cages. The control group had a normal diet and the other feeding conditions were the same. Two groups of newborn rats were given birth. 1 oxygen consumption in air, 15% oxygen concentration, 10% oxygen concentration and 5% oxygen concentration in air was measured by volume drawing method. The oxygen consumption at different oxygen concentrations was compared between the two groups. (2) in vitro medulla oblongata slices were made from neonatal rats for 2 days, and RRDA, was recorded. Use 95% O2 5% carbon dioxide CO2,80%O2 4.2%CO2 15.8% N _ 2N _ 2N _ 2 65 O _ 2 3.4%CO2 31.6in turn and improve kreb's (MKS) from high to high by N250O _ 2 2.6%CO2 47.4%N2 fully saturated at 0 鈩，

本文编号：2429445