AP-2α对滋养细胞凋亡、侵袭作用的影响

发布时间：2019-02-24 12:09

【摘要】：目的研究转录因子激活蛋白-2α(Transcription factor activator protein-2α,AP-2α)对滋养细胞Be Wo凋亡、侵袭等生物学功能的影响,探讨在子痫前期(preeclampsia,PE)发生发展中的作用。方法将已构建的p IRES2-EGFP/AP-2α真核表达载体,采用瞬时转染的方法转入滋养细胞系Be Wo细胞中,应用real timePCR及Western blot方法,检测各组Be Wo细胞中AP-2αm RNA及蛋白表达;采用流式细胞仪检测细胞凋亡,Transwell法检测转染后各组细胞侵袭力。结果 AP-2α转染Be Wo细胞后AP-2αm RNA和蛋白表达量较对照组显著增加,其差异有统计学意义(P0.05)。流式细胞仪检测细胞凋亡结果显示:与对照组比较,AP-2α转染组细胞凋亡数量明显增多,差异有统计学意义(P0.05);Transwell检测细胞侵袭能力实验结果显示:与对照组比较,AP-2α转染组细胞侵袭的数量显著减少,差异有统计学意义(P0.05)。结论证明AP-2α促进滋养细胞凋亡并降低其侵袭力,造成滋养细胞层浅表植入,从而为子痫前期致病机理研究提供了理论依据。
[Abstract]:Objective to investigate the effects of transcription factor activator protein-2 伪 (Transcription factor activator protein-2 伪 (AP-2 伪) on the apoptosis and invasion of trophoblastic Be Wo and its role in the development of preeclampsia (preeclampsia,PE). Methods the constructed eukaryotic expression vector of p IRES2-EGFP/AP-2 伪 was transfected into trophoblastic Be Wo cells by transient transfection. The expression of AP-2 伪 m RNA and protein in Be Wo cells was detected by real timePCR and Western blot methods. Apoptosis was detected by flow cytometry and invasiveness was detected by Transwell assay. Results the expression of AP-2 伪 m RNA and protein in Be Wo cells transfected with AP-2 伪 was significantly higher than that in control group (P0.05). The results of flow cytometry showed that the number of apoptosis in AP-2 伪 transfected group was significantly higher than that in control group (P0.05). Compared with the control group, the invasiveness of AP-2 伪 transfected group was significantly decreased by Transwell (P0.05), and the invasiveness of the transfected group was significantly lower than that of the control group (P0.05). Conclusion AP-2 伪 promotes trophoblast apoptosis and reduces the invasion of trophoblast, which results in superficial implantation of trophoblast layer, which provides a theoretical basis for the study of pathogenesis of preeclampsia.
【作者单位】：郑州大学第三附属医院妇产科;

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