Survivin、CIAPIN1在子宫肌瘤中的表达及意义

发布时间：2019-02-27 07:41

【摘要】：目的：研究Survivn及CIAPIN1在子宫肌瘤中的表达,探讨Surviving CIAPIN1在子宫肌瘤发生中的作用。方法：应用免疫组织化学SP法及实时荧光定量PCR技术分别检测子宫肌瘤和子宫平滑肌组织中Surviving CIAPIN1蛋白及mRNA的表达情况。结果：1.Survivin在子宫肌瘤中的表达 (1) Survivin在肌瘤组织中的阳性表达定位于平滑肌细胞细胞质,偶可见细胞质和细胞核同时染色,Survivin在子宫肌瘤组中的阳性表达率为84.38%,子宫平滑肌组织中阳性表达率为9.68%,两组比较差异具有有统计学意义(x2=36.32,P0.01)。 (2)在子宫肌瘤组、子宫平滑肌组,Survivin mRNA的相对表达量分别为1.03+0.062、0.47±0.039,两组比较差异有统计学意义(P0.05)。 2. CIAPIN1在子宫肌瘤中的表达 (1) CIAPIN1在子宫肌瘤的表达定位于细胞核,细胞浆,细胞核仁中。CIAPIN1在子宫肌瘤组中的阳性表达率为78.13%,子宫平滑肌组织中的的阳性表达率为12.90%,两组比较差异具有统计学意义。(x2=26.96,P0.01) (2)在子宫肌瘤组、子宫平滑肌组,CIAPIN1mRNA的相对表达量分别为1.05±0.057、0.63±0.027,两组比较差异有统计学意义(P0.05)。 3. Survivin及CIAPIN1在子宫肌瘤表达的相关性分析在子宫肌瘤中,Survivin和CIAPIN1蛋白和mRNA均呈高表达,两者在子宫肌瘤中的表达呈现出正相关,相关系数R分别为0.996、0.628差异有统计学意义(P0.05)。结论：1.Survivin及CIAPIN1在子宫肌瘤中的高表达,提示Survivin与CIAPIN1参与子宫肌瘤发生的可能。 2.Survivin、CIAPIN1在子宫肌瘤中的表达呈正相关,提示两者在子宫肌瘤中的发生发展过程中可能存在协同作用。
[Abstract]:Objective: to study the expression of Survivn and CIAPIN1 in uterine leiomyoma and explore the role of Surviving CIAPIN1 in the development of uterine leiomyoma. Methods: immunohistochemical SP method and real-time fluorescence quantitative PCR were used to detect the expression of Surviving CIAPIN1 protein and mRNA in uterine leiomyoma and uterine smooth muscle respectively. Results: the expression of 1.Survivin in leiomyoma (1) the positive expression of Survivin in leiomyoma tissue was localized in smooth muscle cytoplasm and occasionally stained at the same time in cytoplasm and nucleus. The positive expression rate of Survivin was 84.38% in leiomyoma group and 9.68% in uterine smooth muscle tissue. There was significant difference between the two groups (x 2, 36.32, P0.01). (2) in the leiomyoma group, the relative expression of, Survivin mRNA in the leiomyoma group was 1.03,0.062 and 0.47 卤0.039, respectively. There was a significant difference between the two groups (P0.05). 2. Expression of CIAPIN1 in uterine leiomyoma (1) the expression of CIAPIN1 in uterine leiomyoma was localized in nucleus, cytoplasm and nucleolus. The positive expression rate of CIAPIN1 in uterine leiomyoma group was 78.13%, and the positive expression rate of CIAPIN1 in uterine leiomyoma group was 78.13%. The positive rate of expression in uterine smooth muscle tissue was 12.90%, the difference between the two groups was statistically significant. (x2, 26.96, P0.01) (2) in uterine leiomyoma group, uterine smooth muscle group, The relative expression of CIAPIN1mRNA was 1.05 卤0.057 and 0.63 卤0.027, respectively. There was significant difference between the two groups (P0.05). 3. Correlation analysis of Survivin and CIAPIN1 expression in uterine leiomyoma the expression of Survivin and CIAPIN1 protein and mRNA were high in uterine leiomyoma, and the expression of Survivin and CIAPIN1 protein in uterine leiomyoma was positively correlated with that of mRNA in uterine leiomyoma. The correlation coefficient R was 0.996, and 0.628 was statistically significant (P0.05). Conclusion: the high expression of 1.Survivin and CIAPIN1 in uterine leiomyoma suggests that Survivin and CIAPIN1 may be involved in the occurrence of uterine leiomyoma. 2. The expression of survivin and CIAPIN1 in uterine leiomyoma was positively correlated, suggesting that there might be synergetic effect between survivin and CIAPIN1 in the occurrence and development of uterine leiomyoma.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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