产前束缚应激对子代大鼠海马神经干细胞增殖及巢蛋白表达的影响
发布时间：2019-02-27 21:10
【摘要】：为了观察产前束缚应激对子代大鼠空间学习记忆能力、海马神经干细胞增殖及巢蛋白表达的影响,将体重240~260 g的Sprague-Dawley雌性母鼠12只随机分成2组,对照组于孕期不做任何处理,束缚应激组于孕14~20 d时给予束缚应激,3次/天,45 min/次。取1月龄子代大鼠进行实验研究。Morris水迷宫定位航行实验结果显示,应激组子代与对照组相比,到达平台的潜伏期延长(P0.05),而在空间探索实验中,应激组子代在原平台象限停留时间与对照组相比无显著差异。免疫组织化学结果显示,应激组雌性子代海马巢蛋白(nestin)和BrdU阳性细胞表达均较对照组显著增加(P0.05),而雄性子代海马nestin和BrdU阳性细胞表达与对照组相比无显著性差异(P0.05)。以上结果提示,产前束缚应激可引起雌性子代大鼠海马神经干细胞数量增加以及增殖能力增强,可能与机体对产前应激所致脑损伤的代偿性反应相关。
[Abstract]:In order to observe the effects of prenatal restraint stress on spatial learning and memory ability, proliferation of hippocampal neural stem cells (NSCs) and expression of nestin, 12 female Sprague-Dawley female rats weighing 240? 260 g were randomly divided into two groups. In the control group, no treatment was given during pregnancy, while in the restraint stress group, restraint stress was given on the 14th day of gestation, 3 times a day, and 45 min/ times at the 20th day of gestation. Morris water maze navigation experiment showed that the latency of the stress group was longer than that of the control group (P0.05), while in the space exploration experiment, the latency of the stress group was longer than that of the control group (P0.05). There was no significant difference in the retention time of the offspring of the stress group between the original platform quadrant and the control group. Immunohistochemical results showed that the expression of nestin (nestin) and BrdU in hippocampus of female offspring in stress group was significantly higher than that in control group (P0.05). There was no significant difference in the expression of nestin and BrdU positive cells between male offspring and control group (P0.05). These results suggest that prenatal restraint stress can increase the number of neural stem cells and enhance the proliferation of neural stem cells in hippocampus of female offspring, which may be related to the compensatory response of the organism to brain injury induced by prenatal stress.
【作者单位】： 西安医学院第一附属医院妇科;西安医学院第一附属医院药剂科;西安交通大学第二附属医院妇产科;西安交通大学第一附属医院儿科;
【基金】：supported by the Science and Technology Plan Project of the Department of Education of Shaanxi Province,China(No.16JK1643)
【分类号】：R714
本文编号：2431581
[Abstract]:In order to observe the effects of prenatal restraint stress on spatial learning and memory ability, proliferation of hippocampal neural stem cells (NSCs) and expression of nestin, 12 female Sprague-Dawley female rats weighing 240? 260 g were randomly divided into two groups. In the control group, no treatment was given during pregnancy, while in the restraint stress group, restraint stress was given on the 14th day of gestation, 3 times a day, and 45 min/ times at the 20th day of gestation. Morris water maze navigation experiment showed that the latency of the stress group was longer than that of the control group (P0.05), while in the space exploration experiment, the latency of the stress group was longer than that of the control group (P0.05). There was no significant difference in the retention time of the offspring of the stress group between the original platform quadrant and the control group. Immunohistochemical results showed that the expression of nestin (nestin) and BrdU in hippocampus of female offspring in stress group was significantly higher than that in control group (P0.05). There was no significant difference in the expression of nestin and BrdU positive cells between male offspring and control group (P0.05). These results suggest that prenatal restraint stress can increase the number of neural stem cells and enhance the proliferation of neural stem cells in hippocampus of female offspring, which may be related to the compensatory response of the organism to brain injury induced by prenatal stress.
【作者单位】： 西安医学院第一附属医院妇科;西安医学院第一附属医院药剂科;西安交通大学第二附属医院妇产科;西安交通大学第一附属医院儿科;
【基金】：supported by the Science and Technology Plan Project of the Department of Education of Shaanxi Province,China(No.16JK1643)
【分类号】：R714
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