大鼠侧脑室注射瘦素对卵巢摘除给予雌激素子宫腔液2-DE图谱分析

发布时间：2019-04-02 02:38

【摘要】：瘦素(leptin)通过动物下丘脑-垂体轴对其生殖活动予以调节,但瘦素通过下丘脑对子宫调节作用仍不清楚.本研究在大鼠侧脑室微量注射瘦素6 h后,采用双向凝胶电泳(2-DE)结合基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术分离、鉴定了卵巢摘除给予雌激素的子宫腔液蛋白质表达谱.我们获得了分辨率较高、重复性较好的2-DE图谱;质谱分析结合Swiss Prot蛋白数据库检索,共鉴定了24个差异表达的蛋白质与生理盐水注射(对照)比较,侧脑室微量注射瘦素引起22个蛋白质分子显著上调,2个显著下调.重要的是,在上调的蛋白质分子中包括转铁蛋白、基质金属蛋白酶7、膜联蛋白A1、补体B因子、磷酸甘油酸酯变位酶1、烯醇酶、冠蛋白-1A等15个蛋白质分子,下调的2个蛋白质分子是补体C3、溶解物载体家族4成员3.这些蛋白质分子涉及细胞分化、细胞迁移、细胞凋亡,以及炎症反应等功能.实验结果提示,瘦素可能通过下丘脑-垂体轴参与免疫调节、雌性生殖及炎症反应等,从而对子宫发挥调节作用.本研究将为加深对瘦素调节子宫作用的认识,并为深入研究女性生殖系统疾病提供新的启示.
[Abstract]:Leptin (leptin) regulates reproductive activity through the hypothalamus-pituitary axis in animals, but the role of leptin in regulating uterus through hypothalamus is still unclear. Leptin was isolated by two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF-MS) 6 hours after microinjection of leptin into the lateral ventricle of rats. The protein expression profile of uterine fluid after ovariectomy with estrogen was identified. We obtained the 2-DE map with high resolution and good repeatability. Compared with saline injection (control), 24 differentially expressed proteins were identified by mass spectrometry combined with Swiss Prot protein database search. Twenty-two protein molecules were up-regulated and 2 significantly down-regulated by microinjection of leptin into lateral ventricle. Importantly, the up-regulated protein molecules include transferrin, matrix metalloproteinases 7, membranous protein A1, complement B factor, phosphoglycerate transposase 1, enolase, crown-1A, and so on. The down-regulated two protein molecules are complement C3, 4 members of the soluble carrier family 3. These proteins are involved in cell differentiation, cell migration, apoptosis, and inflammatory responses. The results suggest that leptin may be involved in immunomodulation, female reproduction and inflammatory response through hypothalamus-pituitary axis, which may play a regulatory role in uterus. This study will deepen the understanding of the role of leptin in the regulation of uterus, and provide a new inspiration for the further study of female reproductive system diseases.
【作者单位】：承德医学院;
【基金】：河北省自然科学基金项目(No.H2013406115) 河北省卫生厅医学科学研究项目(No.20130012) 河北省人口计生委科学研究项目(No.2012-A22)~~
【分类号】：O657.63;R711

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