外周血β-HCG mRNA表达与妊娠滋养细胞肿瘤早期血行转移的相关研究
发布时间：2019-04-23 08:38
【摘要】：目的： 研究妊娠滋养细胞肿瘤患者外周血β-HCG mRNA表达情况，评价其表达与不同临床指标的关系，，寻找评估妊娠滋养细胞肿瘤早期血行转移的标志物。 方法： 收集28例妊娠滋养细胞肿瘤、15例葡萄胎、10例正常妊娠妇女和10例非妊娠妇女外周血。运用外周血密度梯度离心法提取单个核细胞和滋养细胞后采用RT-PCR方法检测四者β-HCG mRNA表达水平，并与妊娠滋养细胞肿瘤及葡萄胎的临床指标做相关分析。 结果： 1、10例非妊娠妇女外周血β-HCG mRNA表达均阴性。10例正常妊娠妇女外周血中3例见β-HCG mRNA扩增条带，β-HCG mRNA和GAPDH mRNA的灰度比值范围为0.01-0.77。15例葡萄胎患者外周血中8例见β-HCG mRNA扩增条带，β-HCG mRNA和GAPDH mRNA的灰度比值范围为0.02-3.56。28例妊娠滋养细胞肿瘤患者外周血中24例见β-HCG mRNA扩增条带，β-HCG mRNA和GAPDH mRNA的灰度比值范围为0.04-4.56。正常妊娠妇女β-HCG mRNA表达水平低于葡萄胎、妊娠滋养细胞肿瘤患者（P0.05)，但葡萄胎与妊娠滋养细胞肿瘤患者β-HCG mRNA表达水平差异无统计学意义（P0.05)。 2、妊娠滋养细胞肿瘤患者外周血β-HCG mRNA表达与年龄、前次妊娠、距前次妊娠时间、最大肿瘤大小、转移灶数目、预后评分、耐药、化疗疗程及化疗疗效无关（P0.05)，与治疗前血β-HCG水平、肺转移及临床分期有关（P0.05)。随访病例中只有1例进展，无复发病例。目前无法评估外周血β-HCGmRNA与妊娠滋养细胞肿瘤进展及复发的关系。 3、葡萄胎患者外周血β-HCG mRNA表达与治疗前血β-HCG水平、子宫大小、卵巢黄素囊肿直径、第一次清宫后48小时血β-HCG水平及有无恶变均无关（P0.05)。 结论： 1、外周血中单个核细胞不表达β-HCG mRNA。 2、正常妊娠、葡萄胎及妊娠滋养细胞肿瘤均可有滋养细胞进入外周血循环。 3、外周血滋养细胞β-HCG mRNA可能成为评估妊娠滋养细胞肿瘤早期血行转移的有效标志物。
[Abstract]:Aim: to study the expression of 尾-HCG mRNA in peripheral blood of patients with gestational trophoblastic tumor (GTT), evaluate the relationship between the expression of 尾-TNF and different clinical parameters, and find out the markers for evaluating the early hematogenous metastasis of gestational trophoblastic tumor (GTT). Methods: peripheral blood samples were collected from 28 cases of gestational trophoblastic tumors, 15 cases of hydatidiform mole, 10 cases of normal pregnant women and 10 cases of non-pregnant women. Peripheral blood density gradient centrifugation was used to extract mononuclear cells and trophoblasts and then the expression of 尾-HCG mRNA was detected by RT-PCR method. The correlation between 尾-HCG mRNA expression and clinical indexes of gestational trophoblastic tumor and hydatidiform mole was analyzed. Results: 1. The expression of 尾-HCG mRNA in peripheral blood of 10 non-pregnant women was negative, and 尾-HCG mRNA amplification bands were found in 3 of 10 normal pregnant women. The gray scale ratio of 尾-HCG mRNA and GAPDH mRNA was 0.01? 0.77.15 cases of hydatidiform mole, 8 of them showed 尾-HCG mRNA amplification strip. The gray scale ratio of 尾-HCG mRNA and GAPDH mRNA in peripheral blood of 24 patients with gestational trophoblastic tumor was 0.02 ~ 3.56.28, 尾-HCG mRNA amplification band was found in 24 patients, and the gray scale ratio of 尾-HCG mRNA and GAPDH mRNA was 0.04 ~ 4.56. There was no significant difference between 尾-HCG mRNA and GAPDH mRNA in the peripheral blood of patients with gestational trophoblastic tumor. The expression level of 尾-HCG mRNA in normal pregnant women was lower than that in hydatidiform mole, but there was no significant difference in 尾-HCG mRNA expression between hydatidiform mole and gestational trophoblastic tumor (P0.05). 2. The expression of 尾-HCG mRNA in peripheral blood of patients with gestational trophoblastic tumor was not related to age, previous pregnancy, time to previous pregnancy, maximum tumor size, number of metastatic foci, prognosis score, drug resistance, course of chemotherapy and therapeutic effect (P0.05). Serum 尾-HCG level, lung metastasis and clinical stage were correlated with treatment (P0.05). There was only 1 progress in the follow-up cases and no recurrence. It is not possible to evaluate the relationship between 尾-HCGmRNA in peripheral blood and the progression and recurrence of gestational trophoblastic tumors. 3. The expression of 尾-HCG mRNA in peripheral blood of hydatidiform mole patients was not correlated with the level of 尾-HCG, the size of uterus, the diameter of ovarian flavin cyst, the level of 尾-HCG and whether there was malignant change 48 hours after the first uterus clearance (P0.05). Conclusion: 1. There is no expression of 尾-HCG mRNA. in peripheral blood mononuclear cells. In normal pregnancy, hydatidiform mole and gestational trophoblastic tumor, trophoblast cells enter the peripheral blood circulation. 3. Peripheral blood trophoblast 尾-HCG mRNA may be an effective marker for evaluating early blood metastasis of gestational trophoblastic tumor.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
本文编号：2463303
[Abstract]:Aim: to study the expression of 尾-HCG mRNA in peripheral blood of patients with gestational trophoblastic tumor (GTT), evaluate the relationship between the expression of 尾-TNF and different clinical parameters, and find out the markers for evaluating the early hematogenous metastasis of gestational trophoblastic tumor (GTT). Methods: peripheral blood samples were collected from 28 cases of gestational trophoblastic tumors, 15 cases of hydatidiform mole, 10 cases of normal pregnant women and 10 cases of non-pregnant women. Peripheral blood density gradient centrifugation was used to extract mononuclear cells and trophoblasts and then the expression of 尾-HCG mRNA was detected by RT-PCR method. The correlation between 尾-HCG mRNA expression and clinical indexes of gestational trophoblastic tumor and hydatidiform mole was analyzed. Results: 1. The expression of 尾-HCG mRNA in peripheral blood of 10 non-pregnant women was negative, and 尾-HCG mRNA amplification bands were found in 3 of 10 normal pregnant women. The gray scale ratio of 尾-HCG mRNA and GAPDH mRNA was 0.01? 0.77.15 cases of hydatidiform mole, 8 of them showed 尾-HCG mRNA amplification strip. The gray scale ratio of 尾-HCG mRNA and GAPDH mRNA in peripheral blood of 24 patients with gestational trophoblastic tumor was 0.02 ~ 3.56.28, 尾-HCG mRNA amplification band was found in 24 patients, and the gray scale ratio of 尾-HCG mRNA and GAPDH mRNA was 0.04 ~ 4.56. There was no significant difference between 尾-HCG mRNA and GAPDH mRNA in the peripheral blood of patients with gestational trophoblastic tumor. The expression level of 尾-HCG mRNA in normal pregnant women was lower than that in hydatidiform mole, but there was no significant difference in 尾-HCG mRNA expression between hydatidiform mole and gestational trophoblastic tumor (P0.05). 2. The expression of 尾-HCG mRNA in peripheral blood of patients with gestational trophoblastic tumor was not related to age, previous pregnancy, time to previous pregnancy, maximum tumor size, number of metastatic foci, prognosis score, drug resistance, course of chemotherapy and therapeutic effect (P0.05). Serum 尾-HCG level, lung metastasis and clinical stage were correlated with treatment (P0.05). There was only 1 progress in the follow-up cases and no recurrence. It is not possible to evaluate the relationship between 尾-HCGmRNA in peripheral blood and the progression and recurrence of gestational trophoblastic tumors. 3. The expression of 尾-HCG mRNA in peripheral blood of hydatidiform mole patients was not correlated with the level of 尾-HCG, the size of uterus, the diameter of ovarian flavin cyst, the level of 尾-HCG and whether there was malignant change 48 hours after the first uterus clearance (P0.05). Conclusion: 1. There is no expression of 尾-HCG mRNA. in peripheral blood mononuclear cells. In normal pregnancy, hydatidiform mole and gestational trophoblastic tumor, trophoblast cells enter the peripheral blood circulation. 3. Peripheral blood trophoblast 尾-HCG mRNA may be an effective marker for evaluating early blood metastasis of gestational trophoblastic tumor.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
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