卵巢癌细胞株SKOV3中ERβ表达对细胞增殖凋亡影响的研究

发布时间：2019-04-30 07:38

【摘要】：目的:研究卵巢癌细胞株SKOV3中ERβ表达对细胞增殖凋亡的影响。方法:MTT法检测卵巢癌细胞暴露于17β-E2不同浓度及不同时间的细胞增殖率。用特异性雌激素受体激动剂DPN联合17β-E2或单独使用DPN处理细胞,RT-PCR法检测ERβmRNA、ERK1/2 mRNA及caspase-3 mRNA表达,蛋白免疫印记法检测ERβ和p-ERK1/2蛋白表达。结果:10-6mol/L 17β-E2作用60min时,细胞的增殖活性最强(5.7±0.23),与对照组相比(3.5±0.45),差异有统计学意义(P0.05)。ERβ在卵巢癌中低表达。随着DPN作用时间延长及浓度增加,ERβmRNA、caspase-3 mRNA表达逐渐增加(P0.05),ERK2 mRNA表达逐渐降低,与对照组比较差异有统计学意义(P0.05)。随着DPN作用时间延长及浓度的增加,ERβ蛋白表达逐渐增加,p-ERK1/2蛋白表达逐渐降低,与对照组比较差异有统计学意义(P0.05)。结论:ERβ在卵巢癌中低表达,上调ERβ表达能抑制卵巢癌细胞的增殖,同时促进细胞的凋亡。
[Abstract]:Aim: to study the effect of ER 尾 expression on proliferation and apoptosis of ovarian cancer cell line SKOV3. Methods: the proliferation rate of ovarian cancer cells exposed to 17 尾-E _ 2 at different concentration and time was detected by MTT method. The cells were treated with specific estrogen receptor agonist DPN combined with 17 尾-E2 or treated with DPN alone. The expression of ER 尾 mRNA, ERK1/2 mRNA and caspase-3 mRNA was detected by RT-PCR method, and the expression of ER 尾 and p-ERK1/2 protein was detected by immuno-blotting. Results: when 60min was treated with 10-6mol/L 17 尾-E2, the proliferation activity of the cells was the strongest (5.7 卤0.23), compared with the control group (3.5 卤0.45), the difference was statistically significant (P0.05). The expression of ER 尾 in ovarian cancer was lower than that in the control group. With the prolongation of time and concentration of DPN, the expression of ER尾 mRNA and caspase-3 mRNA increased gradually (P0.05), while the expression of ERK2 mRNA decreased gradually (P0.05), there was a significant difference compared with the control group (P0.05). With the prolongation of time and concentration of DPN, the expression of ER 尾 protein increased gradually, and the expression of p-ERK1/2 protein decreased gradually. There was a significant difference compared with the control group (P0.05). Conclusion: the low expression of ER 尾 in ovarian cancer and up-regulation of ER 尾 expression can inhibit the proliferation of ovarian cancer cells and promote the apoptosis of ovarian cancer cells.
【作者单位】：山西省运城市中心医院;
【分类号】：R737.31

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