巨噬细胞表达分泌的抗菌肽在卵巢癌发展中的作用及调控机制
发布时间：2019-05-09 21:43
【摘要】：目的探讨巨噬细胞表达分泌的抗菌肽Hcap18/LL37在卵巢癌发展中的作用及调控机制。方法构建卵巢癌细胞株OVCAR-3与巨噬细胞共培养模型,采用Matrigel小室检测巨噬细胞对OVCAR-3侵袭力的影响;Western印迹法和qRT-PCR检测Hcap18/LL37和Versican V1蛋白和mRNA的表达水平。使用干扰质粒抑制OVCAR-3细胞中Versican V1的表达,分析巨噬细胞中Hcap18/LL37表达和OVCAR-3细胞的侵袭力。结果共培养组侵袭穿膜细胞数明显多于OVCAR-3单独培养组(P0.05);Hcap18/LL37抗体共培养组侵袭穿膜细胞数明显少于对照Ig G共培养组(P0.05)。共培养后巨噬细胞中Hcap18/LL37蛋白和mRNA相对表达量高于单独培养(P0.01);OVCAR-3细胞中Hcap18/LL37蛋白和mRNA相对表达量与单独培养之间差异无统计学意义(P0.05)。Versican V1转染卵巢OVCAR-3细胞与巨噬细胞共培养24 h后,Versican V1蛋白相对表达量高于OVCAR-3细胞单独培养时(P0.01)。巨噬细胞与Versican V1沉默OVCAR-3细胞共培养组侵袭穿膜细胞数低于Versican V1高表达OVCAR-3细胞共培养组(P0.01)。结论卵巢癌内环境中巨噬细胞分泌的抗菌肽Hcap18/LL37促进癌细胞侵袭,其表达受肿瘤细胞分泌的Versican V1蛋白调控。
[Abstract]:Objective to investigate the role and regulatory mechanism of antibacterial peptide Hcap18/LL37 expressed and secreted by macrophages in the development of ovarian cancer. Methods the co-culture model of ovarian cancer cell line OVCAR-3 and macrophage was established. The effect of macrophages on the invasiveness of OVCAR-3 was detected by Matrigel chamber, and the expression of Hcap18/LL37, Versican V1 protein and mRNA was detected by Western and qRT-PCR. The expression of Versican V1 in OVCAR-3 cells was suppressed by interfering plasmid, and the expression of Hcap18/LL37 in macrophages and the invasiveness of OVCAR-3 cells were analyzed. Results the number of invasive transmembrane cells in co-culture group was significantly higher than that in OVCAR-3 alone culture group (P 0.05), and the number of invasive transmembrane cells in Hcap18/LL37 antibody co-culture group was significantly lower than that in control Ig G co-culture group (P 0.05). The relative expression of Hcap18/LL37 protein and mRNA in macrophages after co-culture was higher than that in alone culture (P 0.01). There was no significant difference in the relative expression of Hcap18/LL37 protein and mRNA between OVCAR-3 cells and culture alone (P 0.05). Versican V1 was co-cultured with macrophages for 24 hours). The relative expression of Versican V1 protein was higher than that of OVCAR-3 cells cultured alone (P 0.01). The number of invasive transmembrane cells in the co-culture group of macrophages and Versican V1 silencing OVCAR-3 cells was lower than that in the co-culture group of Versican V1 cells with high expression of OVCAR-3 cells (P 0.01). Conclusion the antibacterial peptide Hcap18/LL37 secreted by macrophages in ovarian cancer environment promotes the invasion of cancer cells, and its expression is regulated by Versican V1 protein secreted by tumor cells.
【作者单位】： 河北医科大学第四医院妇产科;河北医科大学第四医院生殖医学科;
【基金】：河北省卫生和计划生育委员会科研基金项目(No.zl20140305)
【分类号】：R737.31
[Abstract]:Objective to investigate the role and regulatory mechanism of antibacterial peptide Hcap18/LL37 expressed and secreted by macrophages in the development of ovarian cancer. Methods the co-culture model of ovarian cancer cell line OVCAR-3 and macrophage was established. The effect of macrophages on the invasiveness of OVCAR-3 was detected by Matrigel chamber, and the expression of Hcap18/LL37, Versican V1 protein and mRNA was detected by Western and qRT-PCR. The expression of Versican V1 in OVCAR-3 cells was suppressed by interfering plasmid, and the expression of Hcap18/LL37 in macrophages and the invasiveness of OVCAR-3 cells were analyzed. Results the number of invasive transmembrane cells in co-culture group was significantly higher than that in OVCAR-3 alone culture group (P 0.05), and the number of invasive transmembrane cells in Hcap18/LL37 antibody co-culture group was significantly lower than that in control Ig G co-culture group (P 0.05). The relative expression of Hcap18/LL37 protein and mRNA in macrophages after co-culture was higher than that in alone culture (P 0.01). There was no significant difference in the relative expression of Hcap18/LL37 protein and mRNA between OVCAR-3 cells and culture alone (P 0.05). Versican V1 was co-cultured with macrophages for 24 hours). The relative expression of Versican V1 protein was higher than that of OVCAR-3 cells cultured alone (P 0.01). The number of invasive transmembrane cells in the co-culture group of macrophages and Versican V1 silencing OVCAR-3 cells was lower than that in the co-culture group of Versican V1 cells with high expression of OVCAR-3 cells (P 0.01). Conclusion the antibacterial peptide Hcap18/LL37 secreted by macrophages in ovarian cancer environment promotes the invasion of cancer cells, and its expression is regulated by Versican V1 protein secreted by tumor cells.
【作者单位】： 河北医科大学第四医院妇产科;河北医科大学第四医院生殖医学科;
【基金】：河北省卫生和计划生育委员会科研基金项目(No.zl20140305)
【分类号】：R737.31
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