抗神经生长因子抗体用于改善子宫腺肌病小鼠宫腔内环境的研究

发布时间：2019-05-27 12:22

【摘要】：目的:探讨抗神经生长因子抗体(anti-NGF)对改善子宫腺肌病(AM)小鼠宫腔内环境的作用。方法:将15只雌性ICR小鼠随机分为AM治疗组(A组)、AM溶剂对照组(B组)及正常对照组(C组),A、B组使用口服他莫昔芬法建立AM小鼠模型。A组小鼠腹腔注射anti-NGF,B组以等体积的生理盐水替代,C组不作任何处理。3组小鼠于动情前期处死取双子宫称量后检测缓激肽受体1(BKR-1)、神经激肽1受体(NK-1R)、同源框基因转录因子A10(HOXA10)及芳香化酶(aromatase)基因与蛋白的表达。结果:A组子宫相对重量同B组,且均明显高于C组(P0.05);A组BKR-1基因及蛋白和NK-1R基因水平较B组明显降低(P0.05),与C组比较无统计学差异(P0.05);A组HOXA10基因及蛋白水平较B组明显增高(P0.05),与C组比较无统计学差异(P0.05);A组芳香化酶基因及蛋白水平较B组减低(P0.05),但仍高于C组(P0.05)。结论:anti-NGF可显著缓解AM模型小鼠子宫炎症,改善内膜容受性、抑制芳香化酶表达,但短期使用不能抑制病灶增殖。
[Abstract]:Aim: to investigate the effect of anti-nerve growth factor antibody (anti-NGF) on the improvement of uterine cavity environment in (AM) mice with adenomyosis. Methods: fifteen female ICR mice were randomly divided into AM treatment group (group A,), AM solvent control group, group B) and normal control group (group C,), A,). The AM mouse model was established by oral tamoxifen method in group B. the mice in group A were intraperitoneally injected with anti-NGF,B group instead of saline of the same volume. No treatment was performed in group C. the mice in group 3 were killed before estrus and weighing in the uterus. Bradykinin receptor 1 (BKR-1) and neurokinin 1 receptor (NK-1R) were detected. Expression of homeobox gene transcription factor A10 (HOXA10) and aromatase (aromatase) gene and protein. Results: the relative weight of uterus in group A was the same as that in group B, and it was significantly higher than that in group C (P 0.05). The levels of BKR-1 gene and protein and NK-1R gene in group); A were significantly lower than those in group B (P 0.05), but there was no significant difference between group A and group C (P 0.05). The level of HOXA10 gene and protein in group A was significantly higher than that in group B (P 0.05), but there was no significant difference between group A and group C (P 0.05). The level of aromatase gene and protein in group); A was lower than that in group B (P 0.05), but it was still higher than that in group C (P 0.05). Conclusion: anti-NGF can significantly alleviate uterine inflammation, improve intimal receptivity and inhibit aromatase expression in AM model mice, but short-term use can not inhibit the proliferation of lesions.
【作者单位】：上海交通大学医学院附属瑞金医院生殖医学中心;
【基金】：2011年上海高校优秀青年教师培养资助项目
【分类号】：R711.71

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