SCCE、Villin在宫颈腺癌中的表达及意义
发布时间：2019-06-04 22:51
【摘要】：目的：宫颈癌在女性恶性肿瘤中排名第二，基本分为腺癌和鳞癌，宫颈腺癌的发生率要明显低于宫颈鳞癌，据统计显示腺癌约占宫颈癌的5%-8%，但近年来，有研究表明，宫颈腺癌的发病率有所增加，由于宫颈腺癌，不容易被发现等原因，使宫颈腺癌的预后远远差于宫颈鳞状细胞癌，对于宫颈腺癌做出准确的病理诊断对于指导临床尤为重要，本实验主要是研究人角化层糜蛋白酶（stratumcorneum chymotryptic enzyme, SCCE），绒毛蛋白（Villin）在宫颈腺癌发展过程的各阶段中的表达情况，并分析它们对于诊断宫颈腺癌及判断预后的重要意义。 方法：采用免疫组织化学法检测宫颈腺癌45例、宫颈腺上皮内瘤变（cervicalglandular intraepithelial neoplasia, CGIN）24例和正常宫颈组织24例中SCCE，Villin的表达及45例宫颈腺癌中雌激素受体（estrogen receptor，ER）、癌胚抗原（carcino-embryonic antigen，CEA）、波形蛋白（Vimentin）的表达情况，采用Western blotting方法检测10例宫颈腺癌和5例正常宫颈组织中SCCE、Villin的表达情况，结合患者临床病理资料和出院后的随访情况，统计学分析SPSS16.0统计软件包进行。以P0.05为差异有统计学意义。 结果： 1免疫组织化学染色结果 1.1SCCE、Villin在宫颈腺癌、宫颈腺上皮内瘤变和正常宫颈中的表达 对免疫组化结果进行统计学分析：SCCE、 Villin蛋白在宫颈腺癌组、CGIN组中的表达与正常宫颈组的表达比较，进行统计学分析，差异具有显著性（P0.05），宫颈腺癌组与CGIN组间比较，差异无显著性（P＞0.05）。 1.2SCCE、Villin在宫颈腺癌中的表达情况与宫颈腺癌中ER、CEA、Vimentin表达的相关性 对免疫组化结果进行统计学分析：SCCE表达与Villin呈正相关（r=0.434，P0.05)、与Vimentin呈负相关（r=㧟0.373，P0.05)、与ER呈负相关（r=㧟0.629，P0.05)、与CEA表达呈正相关（r=0.434，P0.05)（见表3）；Villin表达与Vimentin呈负相关（r=㧟0.484，P0.05)、与ER呈负相关（r=㧟0.883，P0.05)、与CEA表达呈正相关（r=0.775，P0.05)（见表4）。 1.3SCCE、Villin的表达与宫颈腺癌临床病理参数之间的关系 SCCE、Villin在宫颈腺癌I期中表达率低于II+III+IV期中的表达率，差异有显著性，具有统计学意义（P0.05），在高分化宫颈腺癌中的表达率低于中、低分化宫颈腺癌中的表达率，差异有显著性，具有统计学意义（P0.05），在有淋巴结转移的宫颈腺癌中的表达率高于没有发生淋巴结转移患者的表达率，差异有显著性，具有统计学意义（P0.05）。 1.4宫颈腺癌预后的多因素分析 Cox比例风险模型多因素分析显示：临床分期、组织分级、淋巴结转移情况及SCCE的表达情况可以显著影响宫颈腺癌患者的生存时间（P0.05），但Villin的表达情况对宫颈腺癌患者生存时间的影响不大。 1.5SCCE、Villin蛋白的表达及临床病理参数与宫颈腺癌预后的单因素分析 临床分期I期组，组织分级高分化组，淋巴结未发生转移组，SCCE阴性表达组的生存率显著高于临床分期II+III+IV期组，组织分级中、低分化组，，淋巴结发生转移组，SCCE阳性表达组的生存率，并且差异有显著性，具有统计学意义（P0.05）。 2Western blot结果 SCCE、Villin在宫颈腺癌组中表达率显著高于正常宫颈组（P0.05),差异有显著性，具有统计学意义。 结论 1、SCCE、Villin在正常宫颈、CGIN和宫颈腺癌组中的表达率是呈上升趋势；在宫颈腺癌中，随着临床分期的增加、组织分级的降低及淋巴结转移的发生，SCCE、Villin的表达率都是逐渐升高的，说明SCCE及Villin的表达可能与宫颈腺癌的发生、发展及转移有关。 2、SCCE、Villin、ER、CEA及Vimentin联合检测可以提高宫颈腺癌的诊断准确率。 3、SCCE的表达可以作为判断宫颈腺癌患者预后的指标之一。
[Abstract]:Objective: Cervical cancer is the second in the female malignant tumor, which is mainly divided into adenocarcinoma and squamous cell carcinoma. The incidence of cervical adenocarcinoma is lower than that of the cervical squamous cell carcinoma. According to the statistics, it shows that the adenocarcinoma is about 5% to 8% of the cervical cancer, but in recent years, it has been shown that the incidence of cervical adenocarcinoma has increased. Because cervical adenocarcinoma is not easy to be found, the prognosis of cervical adenocarcinoma is much worse than that of the cervical squamous cell carcinoma. The expression of Villin in the development of cervical adenocarcinoma was analyzed and their significance in the diagnosis of cervical adenocarcinoma and the prognosis was also analyzed. Methods:45 cases of cervical adenocarcinoma,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue were used to detect the expression of SCCE, Villin and the expression of estrogen receptor (ER), carcinoembryonic antigen (CEA) in 45 cases of cervical adenocarcinoma. The expression of Vimentin and the expression of SCCE and Villin in 10 cases of cervical adenocarcinoma and 5 normal cervical tissues were detected by Western blotting. Line. There is a statistical meaning for the difference in the difference of P0.05 Righteousness. Results:1 immune tissue Chemical staining results 1.1 SCCE, Villin in cervical adenocarcinoma, cervical intraepithelial neoplasia and The expression of SCCE and Villin in the cervical adenocarcinoma group and the CGIN group was analyzed by the expression of SCCE and Villin in the normal cervical carcinoma. There was no significant difference between the cervical adenocarcinoma group and the CTIN group, and the difference was not significant between the cervical adenocarcinoma group and the CGIN group. (P> 0.05). 1.2 The expression of SCCE and Villin in the cervical adenocarcinoma and the ER, CEA, Vi in the cervical adenocarcinoma The correlation of mentin expression was found to be positively correlated with Villin (r = 0.434, P0.05), and negative correlation with Vimentin (r = 0.373, P0.05), and negative correlation with ER (r = 0.434, P <0.05). ? 0.629, P0.05), positive correlation with the expression of CEA (r = 0.434, P0.05) (see Table 3), negative correlation with Vimentin (r =-0.484, P0.05), negative correlation with ER (r =-0.883, P0.05), and positive correlation with the expression of CEA (r = 0.77). 5, P0.05) (see Table 4). 1.3 Expression of SCCE and Villin The relationship between the clinicopathological parameters of cervical adenocarcinoma and the expression rate of Villin in the phase I of cervical adenocarcinoma was lower than that in the II + III + IV phase, and the difference was significant (P0.05). The expression rate of the high-differentiated cervical adenocarcinoma was lower than that of the medium and the low-differentiated cervical adenocarcinoma, and the difference was significant (P0.05). The expression rate in the cervical adenocarcinoma with lymph node metastasis was higher than that of the patients without lymph node metastasis. It is of statistical significance (P 0.0). 5).4. The multi-factor analysis of the Cox proportional risk model for the prognosis of cervical adenocarcinoma showed that the clinical stage, the histological grade, the lymph node metastasis and the expression of SCCE could be displayed. The survival time of patients with cervical adenocarcinoma was affected (P0.05), but Vilin The effect of the expression of the expression on the survival time of the patients with cervical adenocarcinoma was not significant. The single-factor analysis of the expression of white and the clinicopathological parameters and the prognosis of cervical adenocarcinoma showed that the survival rate of the SCCE-negative expression group was significantly higher in the stage I, the high-differentiation group, the non-metastasis group and the SCCE-negative expression group. Bed stage II + III + IV group, tissue grade, low differentiation group, lymph node metastasis group, SCCE positive expression group's survival The rate, and the difference is significant, has a series The expression rate of SCCE and Villin in the cervical adenocarcinoma group was significantly higher than that of the Western blot. above Conclusion: The expression rate of SCCE and Villin in the normal cervical, CGIN and cervical adenocarcinoma group is on the rise. In the cervical adenocarcinoma, with the increase of clinical stage, the grade of the tissue is decreased. The expression rate of SCCE and Villin was gradually increased, and the expression rate of SCCE and Villin was gradually increased. The expression of SCCE and Villin may be related to the occurrence, development and metastasis of cervical adenocarcinoma. The combination of n, ER, CEA and Vimentin can improve the cervical adenocarcinoma
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
[Abstract]:Objective: Cervical cancer is the second in the female malignant tumor, which is mainly divided into adenocarcinoma and squamous cell carcinoma. The incidence of cervical adenocarcinoma is lower than that of the cervical squamous cell carcinoma. According to the statistics, it shows that the adenocarcinoma is about 5% to 8% of the cervical cancer, but in recent years, it has been shown that the incidence of cervical adenocarcinoma has increased. Because cervical adenocarcinoma is not easy to be found, the prognosis of cervical adenocarcinoma is much worse than that of the cervical squamous cell carcinoma. The expression of Villin in the development of cervical adenocarcinoma was analyzed and their significance in the diagnosis of cervical adenocarcinoma and the prognosis was also analyzed. Methods:45 cases of cervical adenocarcinoma,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue were used to detect the expression of SCCE, Villin and the expression of estrogen receptor (ER), carcinoembryonic antigen (CEA) in 45 cases of cervical adenocarcinoma. The expression of Vimentin and the expression of SCCE and Villin in 10 cases of cervical adenocarcinoma and 5 normal cervical tissues were detected by Western blotting. Line. There is a statistical meaning for the difference in the difference of P0.05 Righteousness. Results:1 immune tissue Chemical staining results 1.1 SCCE, Villin in cervical adenocarcinoma, cervical intraepithelial neoplasia and The expression of SCCE and Villin in the cervical adenocarcinoma group and the CGIN group was analyzed by the expression of SCCE and Villin in the normal cervical carcinoma. There was no significant difference between the cervical adenocarcinoma group and the CTIN group, and the difference was not significant between the cervical adenocarcinoma group and the CGIN group. (P> 0.05). 1.2 The expression of SCCE and Villin in the cervical adenocarcinoma and the ER, CEA, Vi in the cervical adenocarcinoma The correlation of mentin expression was found to be positively correlated with Villin (r = 0.434, P0.05), and negative correlation with Vimentin (r = 0.373, P0.05), and negative correlation with ER (r = 0.434, P <0.05). ? 0.629, P0.05), positive correlation with the expression of CEA (r = 0.434, P0.05) (see Table 3), negative correlation with Vimentin (r =-0.484, P0.05), negative correlation with ER (r =-0.883, P0.05), and positive correlation with the expression of CEA (r = 0.77). 5, P0.05) (see Table 4). 1.3 Expression of SCCE and Villin The relationship between the clinicopathological parameters of cervical adenocarcinoma and the expression rate of Villin in the phase I of cervical adenocarcinoma was lower than that in the II + III + IV phase, and the difference was significant (P0.05). The expression rate of the high-differentiated cervical adenocarcinoma was lower than that of the medium and the low-differentiated cervical adenocarcinoma, and the difference was significant (P0.05). The expression rate in the cervical adenocarcinoma with lymph node metastasis was higher than that of the patients without lymph node metastasis. It is of statistical significance (P 0.0). 5).4. The multi-factor analysis of the Cox proportional risk model for the prognosis of cervical adenocarcinoma showed that the clinical stage, the histological grade, the lymph node metastasis and the expression of SCCE could be displayed. The survival time of patients with cervical adenocarcinoma was affected (P0.05), but Vilin The effect of the expression of the expression on the survival time of the patients with cervical adenocarcinoma was not significant. The single-factor analysis of the expression of white and the clinicopathological parameters and the prognosis of cervical adenocarcinoma showed that the survival rate of the SCCE-negative expression group was significantly higher in the stage I, the high-differentiation group, the non-metastasis group and the SCCE-negative expression group. Bed stage II + III + IV group, tissue grade, low differentiation group, lymph node metastasis group, SCCE positive expression group's survival The rate, and the difference is significant, has a series The expression rate of SCCE and Villin in the cervical adenocarcinoma group was significantly higher than that of the Western blot. above Conclusion: The expression rate of SCCE and Villin in the normal cervical, CGIN and cervical adenocarcinoma group is on the rise. In the cervical adenocarcinoma, with the increase of clinical stage, the grade of the tissue is decreased. The expression rate of SCCE and Villin was gradually increased, and the expression rate of SCCE and Villin was gradually increased. The expression of SCCE and Villin may be related to the occurrence, development and metastasis of cervical adenocarcinoma. The combination of n, ER, CEA and Vimentin can improve the cervical adenocarcinoma
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
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