脂肪酸结合蛋白4 DNA甲基化在L-NAME干预胎盘滋养细胞脂代谢异常中的作用

发布时间：2019-06-07 17:22

【摘要】：目的观察并分析一氧化氮合酶抑制剂L-NAME对滋养细胞脂肪酸结合蛋白4(FABP4)基因启动子区DNA甲基化及脂质代谢异常的影响。方法体外培养人胎盘滋养细胞(HTR-8)并用100μmol/L-NAME干预48 h后检测胎盘滋养细胞脂质含量;qRT-PCR和Western blot检测胎盘滋养细胞中DNA甲基转移酶1(DNMT1)、FABP4 m RNA和蛋白表达改变;巢式甲基化特异性PCR(NT-MSP)检测胎盘滋养细胞中FABP4启动子区DNA甲基化的程度改变。结果 L-NAME干预滋养细胞后脂质含量明显增多,且细胞内FABP4 mRNA及蛋白表达显著增加(P0.05),而FABP4基因启动子区甲基化程度以及DNMT1的表达水平显著减低。结论 FABP4启动子区低DNA甲基化可能参与了L-NAME诱导的胎盘滋养细胞脂代谢异常的调控过程。
[Abstract]:Objective to observe and analyze the effects of nitric oxide synthase inhibitor L-NAME on DNA methylation and lipid metabolism in the promoter region of trophoblast fatty acid binding protein 4 (FABP4) gene. Methods Human placental trophoblasts (HTR-8) were cultured in vitro and treated with 100 渭 mol / L-NAME for 48 h. The expression of DNA methyltransferase 1 (DNMT1), FABP4 m RNA and protein in placental trophoblast was detected by qRT-PCR and Western blot, and the degree of DNA methylation in FABP4 promoter region in placental trophoblast was detected by nest methylated specific PCR (NT-MSP). Results after L-NAME intervention, the lipid content was significantly increased, and the expression of FABP4 mRNA and protein in the cells was significantly increased (P 0.05), while the degree of methylation in the promoter region of FABP4 gene and the expression of DNMT1 were significantly decreased. Conclusion low DNA methylation in FABP4 promoter region may be involved in the regulation of abnormal lipid metabolism in placental trophoblast induced by L-NAME.
【作者单位】：银川市第一人民医院妇科;宁夏医科大学基础医学院;宁夏医科大学总医院;
【基金】：国家自然科学基金项目(编号:81570452,81660258) 宁夏高等学校科学研究项目(编号:NGY2016086)
【分类号】：R714.246

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