hTERC、ASPP2 mRNA在子宫内膜癌变过程中的表达变化及意义
发布时间：2019-06-16 17:38
【摘要】：目的探讨h TERC、ASPP2 mRNA在子宫内膜癌变过程中的表达变化,并探讨其临床意义。方法收集正常子宫内膜、子宫内膜增生(单纯增生、复合增生、不典型增生)、子宫内膜癌组织,分别采用荧光原位杂交法、半定量RT-PCR法检测h TERC、ASPP2 mRNA,分析二者相关性及其与子宫内膜癌临床病理特征的关系。结果在正常子宫内膜、子宫内膜单纯增生、复合增生、不典型增生及子宫内膜癌中h TERC mRNA表达依次升高、ASPP2mRNA表达依次降低,除h TERC在正常子宫内膜与单纯增生之间、ASPP2 mRNA在不典型增生与子宫内膜癌之间的表达差异无统计学意义(P均0.05)外,其余两两比较差异均有统计学意义(P均0.05);不同子宫内膜组织中h TERC、ASPP2 mRNA的表达呈负相关(r=-0.625,P0.05);h TERC、ASPP2 mRNA表达与子宫内膜癌组织学分级、癌浸润深度、淋巴结转移有关(P均0.05),h TERC mRNA表达与临床分期无关(P0.05),ASPP2 mRNA表达与临床分期有关(P0.05)。结论在子宫内膜癌变过程中,h TERC mRNA表达上调、ASPP2 mRNA表达下调或缺失,二者有助于子宫内膜癌的早期诊断和预后判断。
[Abstract]:Objective to investigate the expression of h TERC,ASPP2 mRNA in endometrial carcinogenesis and its clinical significance. Methods the correlation between normal endometrium, intimal hyperplasia (simple hyperplasia, compound hyperplasia, atypical hyperplasia) and endometrial carcinoma was detected by fluorescence in situ hybridization and semi-quantitative RT-PCR, respectively. The correlation between them and the clinicopathological features of endometrial carcinoma was analyzed by semi-quantitative PCR. Results the expression of h TERC mRNA increased and the expression of ASPP2mRNA decreased in normal endometrium, simple hyperplasia, compound hyperplasia, atypical hyperplasia and endometrial carcinoma. Except for h TERC, there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma, but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and intimal carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between normal and simple hyperplasia (P < 0.05), but there was no significant difference between normal and simple hyperplasia (P < 0.05). The expression of h TERC,ASPP2 mRNA in different endometrial tissues was negatively correlated (r 鈮，
本文编号：2500703
[Abstract]:Objective to investigate the expression of h TERC,ASPP2 mRNA in endometrial carcinogenesis and its clinical significance. Methods the correlation between normal endometrium, intimal hyperplasia (simple hyperplasia, compound hyperplasia, atypical hyperplasia) and endometrial carcinoma was detected by fluorescence in situ hybridization and semi-quantitative RT-PCR, respectively. The correlation between them and the clinicopathological features of endometrial carcinoma was analyzed by semi-quantitative PCR. Results the expression of h TERC mRNA increased and the expression of ASPP2mRNA decreased in normal endometrium, simple hyperplasia, compound hyperplasia, atypical hyperplasia and endometrial carcinoma. Except for h TERC, there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma, but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and intimal carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between atypical hyperplasia and endometrial carcinoma (P < 0.05), but there was no significant difference in the expression of ASPP2mRNA between normal and simple hyperplasia (P < 0.05), but there was no significant difference between normal and simple hyperplasia (P < 0.05). The expression of h TERC,ASPP2 mRNA in different endometrial tissues was negatively correlated (r 鈮，
本文编号：2500703
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