TGF-β1诱导的Postn、α-SMA和Collagen Ⅰ在宫腔粘连内膜组织中表达上调且与粘连程度相关
发布时间：2021-03-05 09:46
目的:骨膜蛋白（Postn）参与多种纤维化疾病的病理形成,且与促纤维化因子TGF-β1的信号通路活动密切相关,但在宫腔粘连（IUAs）形成过程中的作用机制尚不清楚。本研究通过检测不同程度IUAs患者子宫内膜组织中Postn与纤维标志物（α-SMA,CollagenⅠ）的mRNA和蛋白质表达,并从细胞水平研究TGF-β1作用与三种目标蛋白质表达,从而探讨Postn、α-SMA和CollagenⅠ在IUAs发病过程中的作用机制,及与IUAs严重程度间的关系。方法:收集明确诊断的轻、中和重度IUAs患者内膜组织各20例（实验组）,非宫腔粘连者20例（对照组）,利用实时荧光定量PCR技术、Western印迹技术和免疫组化染色分别检测Postn、α-SMA和CollagenⅠ在不同粘连程度IUAs患者的子宫内膜组织中mRNA和蛋白质表达差异。同时,Western印迹技术分别检测原代ESCs经TGF-β1溶液不同时间、不同浓度作用后Postn、α-SMA和CollagenⅠ蛋白表达情况。结果:Postn与α-SMA、CollagenⅠ在IUAs患者子宫内膜组织中的mRNA和蛋白质表达水平趋势一致,...
【文章来源】：重庆医科大学重庆市
【文章页数】：53 页
【学位级别】：硕士
【部分图文】：
qRT-PCR提示Postn、α-SMA和CollagenⅠ在mRNA水平的表达与IUAs粘连程度成正相关Fig2.1ThemRNAexpressionofPostnwaspositivelycorrelatedwiththelevelsofαSMAand
图 2.2 Western blotting 提示 IUAs 患者子宫内膜组织中 Postn、α-SMA 和 CollagenⅠ在蛋白质水平的表达升高Fig 2.2 The protein levels of Postn、α-SMA and Collagen Ⅰ were aberrantly increased in IUAsendometrium.（ A） Total protein was isolated from the endometrium of patients with/withoutIUAs ． The protein levels of Postn 、 α-SMA and Collagen Ⅰ were detected by Westernblotting．GAPDH was used as loading control．（B）Graphic representation of relative protein
图 2.3 IHC 技术示 Postn、α-SMA and Collagen Ⅰ在 IUAs 组呈不同程度的阳性表达（100μm）Fig 2.3The protein levels of Postn, α SMA and Collagen Ⅰ in IUAs and control groups byIHC staining（100μm）. The protein levels of Postn、α-SMA and Collagen Ⅰ were analyzed usingimmunohistochemical staining for the tissues of IUAs and normal endometrium. Brown stainingshowed positive results for Postn(A), α-SMA(B) and Collagen Ⅰ(C). Graphicrepresentation for IODof Postn, α-SMA and Collagen Ⅰ(D). Data were presented as mean ±SD of five independent
【参考文献】：
期刊论文
[1]妇康口服液对大鼠实验性宫腔粘连预防效果及对宫内膜组织TGF-β1、PAI-1和MMP-9表达影响的实验研究[J]. 胡莎,李亚,孟维杰,谭世桥. 四川大学学报(医学版). 2013(04)
[2]Transforming growth factor-β and fibrosis[J]. Franck Verrecchia,Alain Mauviel. World Journal of Gastroenterology. 2007(22)
本文编号：3064996
【文章来源】：重庆医科大学重庆市
【文章页数】：53 页
【学位级别】：硕士
【部分图文】：
qRT-PCR提示Postn、α-SMA和CollagenⅠ在mRNA水平的表达与IUAs粘连程度成正相关Fig2.1ThemRNAexpressionofPostnwaspositivelycorrelatedwiththelevelsofαSMAand
图 2.2 Western blotting 提示 IUAs 患者子宫内膜组织中 Postn、α-SMA 和 CollagenⅠ在蛋白质水平的表达升高Fig 2.2 The protein levels of Postn、α-SMA and Collagen Ⅰ were aberrantly increased in IUAsendometrium.（ A） Total protein was isolated from the endometrium of patients with/withoutIUAs ． The protein levels of Postn 、 α-SMA and Collagen Ⅰ were detected by Westernblotting．GAPDH was used as loading control．（B）Graphic representation of relative protein
图 2.3 IHC 技术示 Postn、α-SMA and Collagen Ⅰ在 IUAs 组呈不同程度的阳性表达（100μm）Fig 2.3The protein levels of Postn, α SMA and Collagen Ⅰ in IUAs and control groups byIHC staining（100μm）. The protein levels of Postn、α-SMA and Collagen Ⅰ were analyzed usingimmunohistochemical staining for the tissues of IUAs and normal endometrium. Brown stainingshowed positive results for Postn(A), α-SMA(B) and Collagen Ⅰ(C). Graphicrepresentation for IODof Postn, α-SMA and Collagen Ⅰ(D). Data were presented as mean ±SD of five independent
【参考文献】：
期刊论文
[1]妇康口服液对大鼠实验性宫腔粘连预防效果及对宫内膜组织TGF-β1、PAI-1和MMP-9表达影响的实验研究[J]. 胡莎,李亚,孟维杰,谭世桥. 四川大学学报(医学版). 2013(04)
[2]Transforming growth factor-β and fibrosis[J]. Franck Verrecchia,Alain Mauviel. World Journal of Gastroenterology. 2007(22)
本文编号：3064996
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