M2型丙酮酸激酶在早孕小鼠子宫内膜的表达

发布时间：2021-09-19 14:08

　　目的成功的胚胎植入是正常妊娠的先决条件。胚泡植入子宫内膜的过程涉及多种激素、细胞因子、粘附分子、免疫系统等的协同作用,与代谢途径也密切相关。糖代谢是三大物质代谢中重要组成部分,组织细胞在有氧条件下进行糖酵解的现象称为瓦氏效应（Warburg effect）。M2型丙酮酸激酶（pyruvate kinase M2,Pkm2）是糖酵解过程中一个标志性的代谢酶,在糖酵解过程中发挥重要作用。已有研究表明在早孕小鼠子宫内膜蜕膜化过程中存在有氧糖酵解这一特殊现象,课题组前期基因芯片筛查结果初步显示Pkm2在早孕D5小鼠子宫内膜的表达呈现着床点高于着床旁的趋势。但是,Pkm2在早孕小鼠子宫内膜的表达规律、与子宫内膜蜕膜化的关系并不清楚。因此,本研究初步探讨Pkm2在早孕小鼠子宫内膜的表达规律,为后续进一步探讨其在早孕小鼠子宫内膜中的作用提供依据。方法（1）建立正常妊娠小鼠模型,分别收集孕D1、D4、D5、D6、D7小鼠子宫内膜组织,采用Real-time PCR、免疫印迹法和免疫组织化学方法,检测Pkm2在早孕小鼠子宫内膜中的表达规律。（2）建立假孕小鼠模型,分别收集假孕PD1、PD4、PD5、PD... 

【文章来源】：重庆医科大学重庆市

【文章页数】：44 页

【学位级别】：硕士

【部分图文】：

Pkm2在早孕小鼠子宫内膜的表达

图 2 Pkm2 在早孕小鼠 D5、D6、D7 子宫内膜着床点及着床旁的表达Fig.2 The result of relative expression of pkm2 protein and mRNA in the endometrium of miceat implantation site and inter-implantation site on D5、D6 and D7.（A-C）Real-time PCR 检测 Pkm2 mRNA 在孕 D5、D6、D7 着床点及着床旁小鼠子宫内膜表达;(D-F)Western blot 检测 Pkm2 蛋白孕 D5、D6、D7 着床点及着床旁小鼠子宫内膜表达;(G)D 图的灰度分析结果;(H)E 图的灰度分析结果;(I)F 图的灰度分析结果。IS:着床点；IIS:着床旁.*P <0.05；**P <0.01；***P <0.001(A-C)Pkm2 mRNA levels determined by Real-time PCR in the endometrium of mice atimplantation site and inter-implantation site on D5 、D6 and D7; (D-F)Pkm2 protein levelsdetermined by Western blot in the endometrium of mice at implantation site andinter-implantation site on D5、D6 and D7; （G）Quantified the levels of Pkm2 protein in imageD; (H)Quantified the levels of Pkm2 protein in image E; (I)Quantified the levels of Pkm2

图 3 Pkm2 蛋白在正常妊娠早孕小鼠子宫内膜的表达Figure 3 The location of Pkm2 protein in the endometrium of mice during early pregnancy.D1、D4：Pkm2 蛋白在小鼠孕第 1、4 天子宫内膜表达；D5IIS、D6IIS、D7IIS：Pkm2 蛋白在小鼠孕第 5、6、7 天着床旁子宫内膜表达；D5IS、D6IS、D7IS：Pkm2 蛋白在小鼠孕第 5、6、7 天着床点子宫内膜表达； CON：阴性对照；Le: 腔上皮；Ge: 腺上皮；S:基质细胞；Em:胚胎；IS:着床点；IIS:着床旁D1、D4: Pkm2 protein location determined by IHC in the endometrium of mice during earlypregnancy; D5IIS 、 D6IIS 、 D7IIS:Pkm2 protein location determined by IHC in theendometrium of mice at inter-implantation site on D5、D6、D7;D5IS、D6IS、D7IS:Pkm2 proteinlocation determined by IHC in the endometrium of mice at implantation site on D5、D6、D7;CON: negative control; Le: luminal epithelium; Ge: gland epithelium; S: stromal cell;Em:embryo; IS:implantation site; IIS:inter-implantation site .

【参考文献】：
期刊论文
[1]Serum M2-pyruvate kinase: A promising non-invasive biomarker for colorectal cancer mass screening[J]. Wen Meng, Hong-Hong Zhu, Ze-Feng Xu, Shan-Rong Cai, Qi Dong, Qiang-Rong Pan, Shu Zheng, Su-Zhan Zhang, Zhejiang University Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences), Hangzhou 310009, Zhejiang Province, China , The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China , Department of Cardiothoracic Surgery, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China, Department of Health Administration, Public Health and Gerontology, School of Public Service Leadership, Capella University, Minneapolis, MN 55403, United States, Department of Community Health, Saint Louis University School of Public Health, Saint Louis, MO 63104, United States , Department of Public Health, College of Health and Human Services, Western Kentucky University, Bowling Green, KY 42101, United States.  World Journal of Gastrointestinal Oncology. 2012(06)
[2]子宫内膜蜕膜化的特征及其调节因素[J]. 韩丙辰,杨增明.  细胞生物学杂志. 2007(05)
[3]子宫内膜胚泡黏附时着床点与着床旁组织的分离[J]. 潘卫,王应雄,黎刚,刘孝云,余秋波.  贵阳医学院学报. 2004(06)
[4]Tumor type M2 pyruvate kinase expression in gastric cancer,colorectal cancer and controls[J]. Bo Zhang Jian-Ying Chen Dao-Da Chen Guo-Bin Wang Department of General Surgery,Affiliated Xiehe Hospital of Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,ChinaPing Shen Department of Biology,Wuhan University,Wuhan 430074,Hubei Province,China.  World Journal of Gastroenterology. 2004(11)



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