金雀异黄酮对体外培养的子宫内膜异位细胞的作用及其机制

发布时间：2022-10-05 16:00

　　目的:探讨金雀异黄酮（Genistein,GEN）对体外培养的小鼠子宫内膜异位细胞的抑制作用及其凋亡相关的机制。方法:选取30只6～7周龄,体重20g的雌性Balb/c小鼠,制备小鼠子宫内膜异位症动物模型,分别从正常小鼠的子宫,异位症小鼠子宫及异位症小鼠的种植物中切取组织块,降解,温育并最终过滤获得内膜细胞,在DMEM培养基中进行原代培养,在电镜下观察其形态及生长模式。不同剂量（0.1 μM,1μM,10 μM）的金雀异黄酮处理正常子宫内膜细胞,异位症在位细胞（EMs Eutopic cells）和异位症异位细胞（EMs Ectopic cells）24小时后,用MTT法测定观察GEN对内膜细胞的抑制作用;采用ANNEXIN V/PI染色法观察了各组内膜细胞的凋亡率;利用PCR技术检测高剂量（10 μM）金雀异黄酮处理前、后异位内膜胞中Caspase-8和NF_κ B的表达。结果:1)体外成功培养了正常子宫内膜、EMs在位内膜及EMs异位内膜细胞;电镜下观察异位症内膜间质细胞较在位间及正常内膜间质细胞偏小,表面不规则,有较多微绒毛。2)正常子宫和EMs在位内膜细胞生... 

【文章页数】：59 页

【学位级别】：硕士

【文章目录】：
摘要
ABSTRACT
LIST OF ABBREBRATION
CHAPTER 1-INTRODUCTION
CHAPTER 2-MATERIALS AND METHODS
    2.1 MATERIALS
        2.1.1 Animals
        2.1.2 Drugs and Reagents
        2.1.3 Instrument
    2.2 METHODS
        2.2.1 Establishing a model of endometriosis
        2.2.2 Obtaining the primary culture
        2.2.3 Cell morphology
        2.2.4 Treatment with different dose of GEN
        2.2.5 Determining cell viability by MTT Assay
        2.2.6 Quantification of apoptotic cell death by Annexin- V FITC/PI assay
        2.2.7 RNA extraction, Reverse transcription and Semi quantitative PCR
        2.2.8 Statistical Analysis
CHAPTER 3-RESULTS
    3.1 GROSS AND HISTOPATHOLOGICAL OBSERVATION
    3.2 GEN REDUCES THE ENDOMETRIOTIC CELL GROWTH IN HIGH DOSE
    3.3 GEN TRIGGERS THE APOPTOTIC CELL DEATH
    3.4 GEN AUGMENTED THE SIGNALING PATHWAYS
CHAPTER 4-DISCUSSION
CHAPTER 5-CONCLUSION
REFERENCES
ACKNOWLEDGEMENT
REVIEW ARTICLE
    References


【参考文献】：
期刊论文
[1]Aberrant control of NF-κB in cancer permits transcriptional and phenotypic plasticity, to curtail dependence on host tissue: molecular mode[J]. Spiros A.Vlahopoulos.  Cancer Biology ＆ Medicine. 2017(03)
[2]Apoptosis and endometrial receptivity: Relationship with in vitro fertilization treatment outcome[J]. Yulia S Antsiferova,Natalya Y Sotnikova.  World Journal of Obstetrics and Gynecology. 2016(01)



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