microRNA-501-5p通过靶向CYLD促进子宫颈癌细胞增殖、迁移和侵袭能力

发布时间：2024-04-11 00:24

　　研究背景:在世界范围内子宫颈癌是一种常见的女性致死性恶性肿瘤,尽管子宫颈癌近年来的发病率和死亡率明显下降,它依旧在女性常见肿瘤中位居第四位。microRNAs(miRNAs)是一类单链非编码小RNA分子,其长度为18-24个碱基,可与靶m RNA通过碱基互补配对相结合,导致靶m RNA降解或者抑制其翻译。在过去的十几年中,在包括子宫颈癌在内的肿瘤领域,越来越多的研究表明miRNA扮演着一个重要调控者的角色。microRNA-501-5p(miR-501)是一种非保守的miRNA,作为肿瘤发生的潜在关键因子而出现。目前的证据表明miR-501在一些恶性肿瘤中过表达,包括肝癌,胃癌和通过促进细胞增殖作为癌细胞起作用的肺癌。此外,miR-501高表达可能是胃癌患者总体生存预测不良的指标。然而,miR-501对宫颈癌的影响和潜在机制尚未探索。CYLD基因位于染色体16q12.1上,编码去泛素化酶。CYLD基因突变与家族性圆筒瘤(FC),多发性家族性毛发上皮瘤(MFT)和多发性骨髓瘤相关。在肾癌,HCC和子宫颈玻璃状细胞癌细胞系中报道了CYLD的拷贝数减少。同时,在结直肠癌,乳腺癌和肺癌中发现抑...

【文章页数】：89 页

【学位级别】：硕士

【文章目录】：
摘要
English Abstract
1 INTRODUCTION
Research justification
2 MATERIALS AND METHODS
    2.1 Materials
        2.1.1 Instruments, Equipments and Reagents
        2.1.2 Cells Line
    2.2 Methods
        2.2.1 Cell Culture
        2.2.2 Cell Transfection
        2.2.3 RNA Extraction
        2.2.4 mi R-501 detection by Taqman probe q RT-PCR
        2.2.5 SYBR Green dye method qRT-PCR detection of CYLD mRNA
        2.2.6 Western Blot analysis
        2.2.7 CCK-8 assay
        2.2.8 Colony formation assay
        2.2.9 Scratch Wound Healing assay
        2.2.10 Transwell migration and invasion assay
        2.2.11 Annexin V/Propidium iodide apoptosis assay
    2.3 Statistical analysis
3 RESULTS
    3.1 Expression of mi R-501 in Hela cells
    3.2 CYLD is downregulated by mi R-501 in cervical cancer cells
    3.3 miR-501 improves cell proliferative potential of cervical cancer via downregulating CYLD
    3.4 miR-501 promotes the migratory and invasive abilities of cervical cancer cells through downregulating CYLD
    3.5 miR-501 inhibits the apoptosis of He La cells mediated by the downregulation of CYLD
4 DISCUSSIONS
Conclusion
References
LITERATURE REVIEW
    1 Cervical Cancer and its molecular origins
    2 MicroRNA
        2.1 Biogenesis
    3 The role of micro RNA in cervical cancer
        3.1 Cell checkpoint and regulation of micro RNAs in cervical cancer
        3.2 The impact of micro RNA in the control of cells proliferation, migration, invasion and apoptosis in cervical cancer
        3.3 miRNAs associated with chemo-resistance in Cervical Cancer
        3.4 microRNA as a biomarker in cervical cancer diagnosis
        3.5 microRNA as a biomarker in cervical cancer prognosis
        3.6 Treatment with micro RNA supplementation
    4 The role of micro RNA-501 in Cancer
    SUMMARY
    REFERENCES
APPENDIX
ACKNOWLEDGMENT
List of Abbreviations
DEDICATION
PUBLICATION



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