肺炎支原体荚膜多糖与DC-SIGN结合并促进IL-10的分泌

发布时间：2018-01-01 18:46

本文关键词：肺炎支原体荚膜多糖与DC-SIGN结合并促进IL-10的分泌　出处：《细胞与分子免疫学杂志》2013年01期 　论文类型：期刊论文

【摘要】：目的研究肺炎支原体(Mp)荚膜多糖(CPS)与树突状细胞(DC)特异性细胞间黏附分子-3-结合非整合素分子(DC-SIGN,CD209)的相互作用及其对未成熟DC(iDC)分泌IL-10和IL-12的影响。方法提取并纯化Mp的CPS和脂质相关膜蛋白(LAMPs),用间接免疫荧光检测CPS与DC-SIGN受体的作用;ELISA法检测CPS和LAMPs作用后iDC所分泌的IL-10,IL-12水平。结果间接免疫荧光可见Mp CPS可结合到DC表面,且DC-SIGN的特异性封闭抗体可阻断CPS与DC的结合;ELISA检测发现CPS与LAMPs共孵育可促进DC分泌IL-10(P0.05),而IL-12的表达水平刺激前后无统计学的差异。结论 Mp的CPS可识别并结合DC的DC-SIGN受体,并促进未成熟DC分泌IL-10。
[Abstract]:Objective to study the relationship between mycoplasma pneumoniae (MP) capsule polysaccharide (CPS) and dendritic cell (DC) -3-binding non-integrin molecule (DC-SIGN). The interaction of CD209) and its effect on the secretion of IL-10 and IL-12 by immature DCI DC.Methods the CPS and lipide-associated membrane protein (Lampps) of MP were extracted and purified. The effect of CPS and DC-SIGN receptor was detected by indirect immunofluorescence. The level of IL-10 IL-12 secreted by iDC after CPS and LAMPs was detected by ELISA method. Results indirect immunofluorescence showed that MP CPS could bind to the surface of DC. The specific blocking antibody of DC-SIGN could block the binding of CPS to DC. ELISA analysis showed that CPS co-incubated with LAMPs could promote IL-10 P0.05 secretion by DC. Conclusion MP CPS can recognize and bind the DC-SIGN receptor of DC and promote the secretion of IL-10 by immature DC.
【作者单位】：南华大学医学微生物学与免疫学教研室;包头市中心医院检验科;南华大学附属第一医院;
【基金】：国家自然科学基金(81072418)
【分类号】：R392.12
【正文快照】： 树突状细胞(dendritic cell,DC)是目前已知的功能最强的专职性抗原提呈细胞,近年来的研究发现DC表面有一种C型凝集素样受体,即树突状细胞特异性细胞间黏附分子-3-结合非整合素分子[dendritic cell-spe-cific intercellular adhesion molecule-3-grabbing noninte-grin,DC-SIGN

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