人血小板表面CD36糖蛋白检测方法的建立及初步应用

发布时间：2018-03-14 03:51

  本文选题：血小板糖蛋白IV　切入点：CD缺失　出处：《中国实验血液学杂志》2013年04期 　论文类型：期刊论文

【摘要】：个体血小板表面CD36抗原缺乏在随机输注时有产生抗-CD36免疫反应的风险,是血小板输注无效的原因之一。本研究应用流式细胞术检测杭州地区单采血小板供者的血小板表面CD36抗原表达情况,并分析个体血小板上CD36缺失表型的频率。留取献血者新鲜抗凝血样,经离心获取富血小板血浆,洗涤并调整血小板计数至1×106。采用CD36-FITC、CD41-PE单克隆抗体和血小板孵育反应,然后用流式细胞仪检测和分析血小板表面糖蛋白CD36抗原表达情况。对于血小板表面CD36抗原阴性的标本,进一步筛查其单核细胞表面CD36的表达情况。结果表明:192例无偿献血者筛查出7例血小板表面CD36抗原阴性,CD36缺失型频率为3.6%,均为Ⅱ型缺失。人群中个体CD36抗原表达强度存在差异,参照CD36几何平均荧光强度数值大小,59例为低表达,126例为高表达。结论:人群中存在CD36Ⅱ型缺失表型,这些数据将为研究CD36抗原分布提供参考,有助于解决血小板输注无效问题。
[Abstract]:Individual platelet surface CD36 antigen deficiency is at risk of anti-CD36 immunoreaction during random infusion. In this study, flow cytometry was used to detect the expression of CD36 antigen on platelet surface of single platelet donors in Hangzhou area. The frequency of CD36 deletion phenotype on individual platelets was analyzed. Fresh anticoagulant blood samples were collected from blood donors, platelet-rich plasma was obtained by centrifugation, platelet count was washed and adjusted to 1 脳 106. Monoclonal antibody CD36-FITC+ CD41-PE and platelet incubation reaction were used. Then flow cytometry was used to detect and analyze the expression of glycoprotein CD36 antigen on platelet surface. The results showed that the frequency of CD36 antigen negative CD36 deletion in 7 platelets was 3.6, all of them were type 鈪，

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