四逆汤对同型半胱氨酸损伤的EAhy926细胞caveolin-1和eNOS表达的影响

发布时间：2018-03-26 02:53

本文选题：四逆汤　切入点：内皮细胞　出处：《中国病理生理杂志》2013年09期

【摘要】：目的:探讨四逆汤(Sini decoction,SND)防治内皮细胞损伤的机制及陷窝蛋白1(caveolin-1)和一氧化氮(NO)系统在其中的作用。方法:建立同型半胱氨酸(Hcy)损伤的人脐静脉内皮融合细胞(EAhy926细胞)模型,观察四逆汤的保护作用及其对NO系统和caveolin-1的影响。结果:Hcy加入后细胞生长缓慢,贴壁细胞数明显减少,NO浓度明显减低(P0.05),caveolin-1 mRNA和蛋白表达明显增强(P0.05),内皮型NO合酶(eNOS)mRNA和蛋白表达明显减弱(P0.05);四逆汤处理组贴壁细胞及细胞形态明显改善,NO浓度较Hcy模型组明显升高(P0.05),caveolin-1 mRNA和蛋白表达较Hcy模型组明显减弱(P0.05),eNOS mRNA和蛋白表达较Hcy模型组明显增强(P0.05),其中以四逆汤1.0 kg/L+Hcy 4.0μmol/L组最明显。结论:Hcy可能通过增加caveolin-1表达和抑制eNOS表达而损伤人脐静脉内皮细胞,而四逆汤通过抑制caveolin-1表达和增加eNOS的表达拮抗Hcy对细胞的损伤。
[Abstract]:Objective: to investigate the mechanism of Sini decoctionn SND in the prevention and treatment of endothelial cell injury and the role of caveolin-1) and nitric oxide (no) system in the prevention and treatment of endothelial injury. Methods: a model of human umbilical vein endothelial fusion cell line EAhy926 induced by Hcyine homocysteine injury was established. To observe the protective effect of Sini decoction and its effect on no system and caveolin-1. The number of adherent cells significantly decreased the concentration of no decreased significantly, the expression of mRNA and protein increased significantly, the expression of endothelium-type no synthase (ENOSN) mRNA and protein decreased significantly (P0.05), and the adherent cells and cell morphology of Sini decoction group significantly improved the concentration of no compared with Hcy model. The expression of mRNA and protein was significantly increased in group A than that in group Hcy. Conclusion the expression of P0.05Enos mRNA and protein in group A is significantly lower than that in group Hcy, especially in the group of 1.0 kg/L Hcy 4.0 渭 mol/L with Sini decoction. Conclusion it may be possible to increase the expression of caveolin-1 and inhibit the expression of eNOS in Sini decoction (1.0 kg/L Hcy 4.0 渭 mol/L). Expression and damage human umbilical vein endothelial cells, Sini decoction antagonized the damage of Hcy by inhibiting the expression of caveolin-1 and increasing the expression of eNOS.
【作者单位】：中山大学附属第三医院心血管内科;中山大学中西医结合研究所;
【基金】：广东省科技计划(No.2010B080701042)
【分类号】：R363.21

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