体内移植羊膜对家兔肌腱损伤模型肌腱愈合的影响

发布时间：2018-04-12 13:06

本文选题：羊膜 + 跟腱　；参考：《中国生物医学工程学报》2013年04期

【摘要】：研究的目的在于观察羊膜体内移植对肌腱损伤修复的影响。60只跟腱损伤家兔动物模型采用同体对照动物实验方法,大体观察伤口愈合、肌腱粘连及肌腱肥大情况,并分别于术后第2、4、6周等3个时间点取跟腱,HE染色观察损伤区域炎细胞浸润和成纤维细胞数量,免疫组化染色观察胶原纤维排列塑形状况,拉伸试验测定肌腱弹性模量。另取4只家兔不做肌腱切断术作为正常对照,在第0周测定肌腱弹性模量。全部实验动物皮肤切口在术后1周内基本愈合,未发生伤口感染情况。羊膜实验组跟腱粘连动物数量和肌腱断裂区域肥大程度低于阴性对照组(P0.05)。术后第2周,两组动物炎症反应均达到最高值,但羊膜实验组炎细胞(包括中性粒细胞和单核细胞)浸润数量低于阴性对照组(P0.05)。两组实验动物样本中成纤维细胞数量和胶原纤维积累数量无明显差异(P0.05),但羊膜实验组成纤维细胞和胶原纤维排列更具有序性,尤其是在术后第6周,明显好于阴性对照组。术后第2周和第4周,羊膜实验组肌腱弹性模量均优于阴性对照组(P0.05)。羊膜具有促进肌腱早期愈合和抗炎、抗粘连作用。
[Abstract]:The purpose of this study was to observe the effect of amniotic membrane transplantation on the repair of tendon injury. Sixty rabbit models of Achilles tendon injury were used to observe the wound healing, tendon adhesion and tendon hypertrophy.The HE staining of Achilles tendon was used to observe the infiltration of inflammatory cells and the number of fibroblasts in the injured area, the arrangement and shape of collagen fibers were observed by immunohistochemical staining, and the elastic modulus of tendons was measured by tensile test.The elastic modulus of tendon was measured at 0 th week in 4 rabbits without tendon amputation as normal control.All experimental animal skin incisions healed basically within 1 week after operation, and no wound infection occurred.The number of achilles tendon adhesions and the degree of hypertrophy of tendon rupture in the amniotic membrane group were lower than those in the negative control group (P 0.05).At the second week after operation, the inflammatory reaction reached the highest level in both groups, but the infiltration of inflammatory cells (including neutrophils and monocytes) in the amniotic membrane experimental group was lower than that in the negative control group (P 0.05).There was no significant difference in the number of fibroblasts and the accumulation of collagen fibers between the two groups, but the arrangement of fibroblasts and collagen fibers in the amniotic membrane experimental group was more orderly than that in the negative control group, especially at the 6th week after operation.At the 2nd and 4th week after operation, the elastic modulus of tendon in the amniotic membrane group was better than that in the negative control group (P 0.05).Amniotic membrane has the function of promoting early healing, anti-inflammation and anti-adhesion of tendon.
【作者单位】：南昌大学基础医学院;江西师范大学生命科学学院;永修县中医医院;江西瑞济生物工程技术有限公司;
【基金】：国家中小企业创新基金(06C26213601339)
【分类号】：R-332

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