黄连解毒汤加减方对大鼠痔相近动物模型的影响及作用机制研究

发布时间：2018-05-15 21:11

本文选题：黄连解毒汤加减方 + 痔急性期相近模型　；参考：《西南医科大学》2017年硕士论文

【摘要】：目的:通过观察黄连解毒汤加减方熏洗对大鼠痔相近动物模型一般情况、肛周溃疡情况、肛周病理组织形态及诱导型一氧化氮合酶(iNOS)、血管内皮生长因子(VEGF)、前列腺素E2(PGE2)的影响,探讨黄连解毒汤加减方治疗大鼠痔相近动物模型的疗效及作用机制,为痔急性期治疗提供非手术选择及黄连解毒汤加减方在临床中使用提供动物实验依据。方法:准备SD大鼠44只,采用肛周注射冰醋酸法复制痔急性期大鼠相近模型,从中随机选取4只观察肛周溃疡病理切片以确认造模是否成功。然后将余下40只大鼠随机分为实验H1组(高剂量)、实验H2组(低剂量)、实验对照H3组(马应龙痔疮膏)及模型对照H4组(蒸馏水),共4组,每组10只。实验H1组给予黄连解毒汤加减方高浓度熏洗,实验H2组给予黄连解毒汤加减方低浓度熏洗,实验对照H3组给予马应龙痔疮膏涂抹,模型对照H4组给予无菌蒸馏水熏洗,以上各组每日给药2次,间隔4h,治疗周期均为7天。给药期间观察大鼠的一般情况,同时于第2天、第4天、第7天观察肛周溃疡面积、肿胀程度并进行记录。给药结束后,取肛周溃疡组织进行病理学观察并采用免疫组化分析肛周溃疡组织iNOS、VEGF、PGE2阳性率。结果:1.造模结束后各组大鼠肛周观察指标对比:(1)各组大鼠一般情况良好。(2)实验动物模型局部炎症评分、肛周局部标本病理检测均符合痔急性期表现。(3)造模结束后,实验H1组、实验H2组、实验对照H3组及模型对照H4组于用药前局部炎症评分值及溃疡面积统计值都无统计学意义(p0.05),具有可比性。2.给药结束后各组大鼠肛周溃疡面积对比:(1)高浓度汤剂、低浓度汤剂促进肛周局部溃疡面积愈合的作用明显优于马应龙痔疮膏(p0.05);(2)高浓度汤剂和低浓度汤剂对肛周局部溃疡愈合的作用在统计学上无明显的差异(p0.05)。3.给药结束后各组大鼠肛周溃疡病理学检查对比:高浓度汤剂、低浓度汤剂肛周局部溃疡病理学改变评分与马应龙痔疮膏均具有统计学意义(p0.05)。4.给药结束后各组大鼠肛周溃疡组织iNOS、VEGF、PGE2对比:(1)高浓度汤剂、低浓度汤剂抑制iNOS、VEGF、PGE2表达的作用都优于马应龙痔疮膏的抑制作用(P0.05);(2)低浓度汤剂和高浓度汤剂抑制i NOS、VEGF、PGE2表达的作用在统计学上无明显的差异(p0.05)。结论:1.黄连解毒汤加减方能改善大鼠痔相近动物模型的肛周局部的水肿、溃疡症状,且本实验中高、低剂量汤剂对大鼠痔相近模型症状改善效果差别不大;2.黄连解毒汤加减方可改善大鼠痔相近模型肛周局部病理症状,具有抑制炎症反应作用和促进溃疡愈合的作用;3.黄连解毒汤加减方对动物模型痔急性期治疗的作用机制可能与iNOS、VEGF、PGE2三者因子的参与有关;4.本实验证实黄连解毒汤加减方熏洗法对大鼠痔相近模型疗效确切,可为临床上痔病的治疗提供一种非手术治疗方法。
[Abstract]:Objective: to observe the effects of Huanglian jiedu decoction fumigation and washing on rat hemorrhoids similar animal model, perianal ulcer, perianal histopathology and inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF) and prostaglandin E _ 2 (PGE _ 2). To explore the therapeutic effect and mechanism of Huanglian jiedu decoction in treating rat hemorrhoid model, to provide non-operative choice for acute hemorrhoids treatment and to provide animal experimental basis for clinical use of Huanglian jiedu decoction. Methods: Forty-four Sprague-Dawley rats were randomly selected to observe the pathological sections of perianal ulcer in order to confirm whether the model was successful or not. Then the remaining 40 rats were randomly divided into experimental H1 group (high dose group), experimental H2 group (low dose group), experimental control group H3 group (Ma Yinglong hemorrhoid ointment) and model control group H4 (distilled water group, 10 rats in each group). Experimental group H1 was treated with high concentration fumigation of Huanglian jiedu decoction, experimental group H2 was given low concentration of Huanglian jiedu decoction, experimental control group H3 was smeared with Ma Yinglong hemorrhoid ointment, model control group H4 was fumigated with aseptic distilled water. The above groups were given 2 times daily for 4 hours, and the treatment cycle was 7 days. The general condition of rats was observed during administration, and the area of perianal ulcer and the degree of swelling were observed and recorded on day 2, day 4 and day 7. After administration, the tissue of perianal ulcer was taken for pathological observation and the positive rate of iNOS VEGFN PGE2 in perianal ulcer tissue was analyzed by immunohistochemistry. The result is 1: 1. Comparison of perianal observation indexes of rats in each group after model making: (1) the rats in each group were generally in good condition. 2) the local inflammation score of experimental animal model, the pathological examination of perianal local specimen all accord with hemorrhoids in acute phase, and the experimental H1 group was treated with H1 group after the model was made. Experimental H2 group, experimental control H3 group and model control H4 group had no significant difference in local inflammatory score and ulcer area statistical value before medication. The perianal ulcer area of rats in each group was compared with that of the control group after administration. The effect of low concentration decoction on the healing of perianal local ulcer area was significantly better than that of Ma Ying long hemorrhoids ointment (p0.05P0. 05) 2) the effect of high concentration decoction and low concentration decoction on the healing of perianal local ulcer was not statistically significant difference (p 0. 05. 3). Comparison of pathological examination of perianal ulcer in rats of each group after administration: the pathological changes of perianal ulcer of high concentration decoction and low concentration decoction and Ma Yinglong hemorrhoid ointment all had statistical significance (P 0.05). 4. After administration, the rats in each group had perianal ulcer tissue (iNOS) and VEGFGE-2 (contrast with 1: 1) high concentration decoction. The inhibitory effect of low concentration decoction on the expression of iNOS VEGFN PGE2 was better than that of Ma Yinglong hemorrhoid ointment (P 0.05). There was no statistical difference between low concentration decoction and high concentration decoction in inhibiting the expression of iNOS VEGFN PGE2. There was no statistically significant difference in the effect of low concentration decoction and high concentration decoction on the expression of iNOS VEGFN PGE2. Conclusion 1. Huanglian jiedu decoction can improve the symptoms of edema and ulcer around the anus of rat hemorrhoids similar animal model, and there is no significant difference in the effect of high and low dosage decoction in improving symptoms of similar model of hemorrhoids in rats. Huanglian jiedu decoction can improve the perianal pathological symptoms of rat hemorrhoid model and has the effect of inhibiting inflammatory reaction and promoting ulcer healing. The mechanism of Huanglian jiedu decoction in treating hemorrhoids in acute stage may be related to the involvement of iNOS VEGFN PGE2 in the treatment of hemorrhoids. This experiment proved that Huanglian jiedu decoction fumigation washing method for rat hemorrhoids similar model is effective and can provide a non-operative treatment for hemorrhoids.
【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

【参考文献】